Dilution Problem.A culture of Staphylococcus is diluted as follows:(1) 70mL are added to 330mL of water.(2) 100uL from (1) are then added to 9.9mL of water.(3) A 10-3 dilution is made from tube # (2).(4) 10uL from (3) are plated for a pour plate and incubated.There were 80 colonies counted on one half of the plate following incubation.a) What was the overall dilution?b) How many cfu/mL were present in the original culture?c) How many milliliters of water is needed to make a 10-1 dilution using 100 uL from the original culture?

Staphylococci are gram-positive aerobic organisms that belong to the family Staphylococcaceae. These…

Answered: Dilution Problem. A culture of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Question

Dilution Problem.
A culture of Staphylococcus is diluted as follows:
(1) 70mL are added to 330mL of water.
(2) 100uL from (1) are then added to 9.9mL of water.
(3) A 10^-3 dilution is made from tube # (2).
(4) 10uL from (3) are plated for a pour plate and incubated.

There were 80 colonies counted on one half of the plate following incubation.

a) What was the overall dilution?
b) How many cfu/mL were present in the original culture?
c) How many milliliters of water is needed to make a 10^-1 dilution using 100 uL from the original culture?

Expert Solution

Trending now

This is a popular solution!

Step by step

Solved in 4 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions