Describe three major differences between leading and lagging strand synthesis. What feature of DNA polymerase results in the leading and lagging strands being synthesized differently

What are the three major requirements of replication?

DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MER-minus strain of yeast does not have a functional  mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MER-minus and wildtype yeast? More than one answer is correct. Options:   More point mutations will arise in MER-minus yeast.   Fewer point mutations will arise in MER-minus yeast as compared with wildtype.   Of the total point mutations that…

if you have the following sequence of DNA 5' ATTGCGGAGCCTCGAT 3' do the following:

List at least three different types of DNA repair and briefly explain how each is carried out.

The leading and lagging strands of replicating DNA are growing in opposite directions; however, the extension of any DNA strand is always   Question 10 options:   a)  5' to 3'.   b)  3' to 5'.   c)  either 5' to 3' or 3' to 5' depending on the direction of the replication fork.   d)  alternating between 5' to 3' and 3' to 5'.   e)  none of the above.

, describe the normal role of the component, and choose from the selection the most direct effect of the mutation and explain WHY. Choose from the 4 following effects: Initiation of DNA synthesis           okazaki fragment synthesis Leading strand elongation            lagging-strand completion --> Extracts from a strain of bacteria lacking the 5’ to 3’ exonuclease activity of DNA polymerase

Which of the following statements most accurately describes the action of the enzyme RNA polymerase?Select one  1.) RNA polymerase will transcribe only the exons by skipping over the introns within a eukaryotic gene sequence 2.) RNA polymerase will transcribe both DNA strands, moving in the 3' to 5' direction for one strand and 5' to 3' on the other 3.) RNA polymerase will transcribe both DNA strands, but only one RNA molecule will be used during translation 4.)None of the statements accurately describe the function of RNA polymerase

What is the difference in DNA replicatoin on the leading strand versus the lagging strand?

1) The function of ligase is to seal nicks in the backbone of a DNA strand. The function of AP endonuclease is to create a nick in the backbone of a DNA molecule adjacent to an apurinic site, which allows DNA polymerase II access to the DNA to repair the damage and prevent a mutation resulting from the use of a damaged or erroneous strand of DNA as template during DNA replication. Why doesn't ligase simply seal up the nicks the AP endonuclease introduces before DNA pol II can do anything?