Answered: Define the principle and step by step…

Phlebotomy Essentials

6th Edition

ISBN:9781451194524

Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Chapter1: Phlebotomy: Past And Present And The Healthcare Setting

Section: Chapter Questions

Problem 1SRQ

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4. TLC is still useful in the lab but is being replaced by automated chromatographic methods. For example, TLC, FPLC, and HPLC are now automated processes, and computers can control various parameters, such as flow rate, blends of solvent mixtures, etc. Can you think of how the ability to manipulate the parameters of these automated chromatographic methods (sample size, column plate length, flow rate, solvent gradients, etc.) might accentuate or improve an investigator's ability to separate amino acids or other biomolecules?
5. Provided with the following data, compute the corresponding CFU/ml of the original culture. Assume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 104 10-5 Plate 1 ΤNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 ΤNTC 280 90 22 2
5. Complex and enriched media are most ideal to identify specific microbes from a mixed population. True or False
3.
6. Which of the following statement is the most appropriate for the description of a sample which has been filtered with 0.2 um syringe filter A. The sample is sterile B. All bacteria in the sample are removed C. Most of the cells or substances larger than 0.2 um are removed D. The sample was cleaned
3. If we are assuming 1 x 109 cells/mL for the overnight culture and knowing that a reliable countable number of colonies is between 20-200, why has the previous question also asked you to calculate a final concentration of 2 colonies and 2000 colonies?
4. A medium containing an antibiotic is a selective medium. True or False.
1. Name the bio instrument that performs the following function: b. Oxygen Saturation measurement
1. The possible problems 2. The possible solutions
1. your Sample has of 1010 bacteroia per ml and a dilution factor of 108. The spread plate you created had 75 CF. What is teh total count, viable count and %viability ?
6. A patient is to be given 100mg of an antibiotic every 6 hours. It is available in 25mg in 5mls. How many mls should the patient be given in 12 hours?
1. When performing a bacterial lab, what could happen that could lead to incorrect results (potential pitfalls during the lab?)

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