Define the following terms: a. replication b. semiconservative c. replication factory d. primosome e. clamp loader

You are studying RNA viruses and have discovered a new one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don’t know if it is plus or minus stranded. Your lab-mate says, “Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome.” You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate’s suggestion?

Which of the following statements BEST DESCRIBES the main findings of the Meselson-Stahl experiment?   A. DNA can be separated using centrifugation   B. The semiconservative model of DNA replication is more accurate than the dispersive or conservative models of DNA replication   C. Using 14N in experiments is an effective way of tracking nitrogen molecules   D. Bacteria grown in the presence of a heavier nitrogen isotope (15N) will replicate at a slower rate than those that utilise a lighter nitrogen isotope (14N)   E. Both strands of each new DNA double helix are brand new and synthesized from individual nucleotides

In the diagram below, the arrow indicates the direction of movement of a replication fork. O Top; Top (a) Which template strand (top or bottom) is the lead strand? (b) Which strand (top or bottom) would you expect to find the Okazaki fragments? O Bottom; Top Replication fork Bottom; Bottom O Top; Bottom NK Unwinding. ?