The plasmid (episome) F factor mediates which of the following? a. Transduction b. Transformation c. Conjugation d. Replication e. Translocation

Read the statements below and determine which f the recombination mechanisms that it matches with - there could be more than one match or there could be no match at all. For each box, place a YES or NO in the box. Statement 1. Donor cell produces a pilus in order to exchange DNA with recipient cell. 2. Mediated by a bacteriophage. 3. Always involves a plasmid. 4. Allows recombination of chromosomal DNA fragments from donor to recipient. 5. Requires rolling circle replication. 6. Requires donor to recipient cell direct contact. 7. Allows the uptake of free DNA fragments from the environment. 8. Is always horizontal gene exchange. 9. Allows movement of genes from a plasmid to a chromosome. 10. Requires a donor cell to infect a recipient cell in order for recombination to occur. 11. Involves plasmid integration into the chromosome prior to recombination. 12. Involves incorrect packaging of donor DNA into a phage particle in order for recombination to occur. 13. Involves DNA that was…

Nevirapine, an antiretroviral drug used to treat HIV acts as an uncompetitive inhibitor of the viral enzyme, reverse transcriptase (RT). Where does this inhibitor bind to prevent viral replication? Select one: a. The enzyme-substrate complex b. The viral DNA product c. Directly to reverse transcriptase d. The substrate Humans are able to break down amino acids, removing the amino group from the carbon skeleton and excreting waste as urea. Select the correct sequence of events in this metabolic pathway. Select one: a. Transamination, ammonia assimilation, deamination, ammonia metabolism b. Deamination, transamination, ammonia metabolism, ammonia assimilation c. Deamination, ammonia assimilation, transamination, ammonia metabolism d. Transamination, deamination, ammonia assimilation, ammonia metabolism

A small nonessential circular DNA molecule in prokaryotes is called a: a. plasmid b. telomere c. bacteriophage d. prophage

During conjugation, an enzyme callled _______ will nick one strand of the F plasmid at the ______ site after which the ssDNA moves to the recipient cell. The original cell ____________ the F plasmid in this process. Select one: a.endonuclease/ OriT/Loses  b.ligase/ OriC/ Loses c.endonuclease/ OriT /Maintains d.HaeIII/ OriC /Maintains e.None of the Above

Which of the following is the most accurate description of the E.coli replisome? a. Clamp loader positions two Pol III core polymerases on the leading strand and one Pol III core polymerase on the lagging strand b. Single strand template DNA forms two loops on the lagging strand while two Pol III core polymerases synthesize Okazaki fragments c. Okazaki fragments are synthesized in the opposite direction to movement of the replication fork d. Clamp loader only connects with Pol III core polymerases associated with the lagging strand e. Both b. and c. are accurate

Replisomes facilitate replication by looping the lagging strand to accomodate the direction of synthesis.   a. True   b. False

Define the following terms: a. replication b. semiconservative c. replication factory d. primosome e. clamp loader

1. Scientists were able to develop transgenic bacteria that can be used for mercury remediation.  This process involved combining genes for mercury resistance (so that the bacteria itself has protection) and mercury accumulation (thereby allowing the bacteria to sequester Hg for retrieval later on). They probably used the following techniques in creating the transgenic bacteria EXCEPT a. plasmids b. DNA ligase c. DNA polymerase d. restriction enzymes   2. Consider these two parents (Mom and Dad) and their son, Calvin.  (see pic attached). Which two members of this family would show the greatest difference in their DNA profile?

The process by which thymine dimers are directly repaired with the help of visible light is called Select one: a. photosynthesis b. excision repair c. recombination d. photoreactivation