Compound X was developed on a TLC plate using a given eluent. The compound had a Rf value of 0.93 and the solvent front is 10.1 cm from the origin. Using this information, calculate the distance in cm that compound X traveled from the origin. Report your answer to one decimal place. cm

A standard sample of compound A was placed on a silica TLC plate. After elution in 30/70 ethyl acetate/hexane, the sample showed a single spot at 3.4 cm (relative to the initial point) after the solvent had risen to 7.1 cm (relative to the initial point). A sample which was thought to contain this compound was analyzed by TLC and showed two spots after elution in the same solvent system. The solvent height for this plate was 6.5 cm (relative to the initial point) and the spots were located at 3.6 cm and 5.2 cm (relative to the initial point). Can either of these spots possibly be compound A? Explain. Include a discussion of the R, for each spot.

How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?

a. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? Consider the purity of each fraction. - - - - - - - - ----| 1 2 3 4 5

There were 2 spots on the TLC that did not match up with any of the standards. What were the Rf values of these spots? Identify what these chemical species might be and draw their structures. Solvent was 3:1:1 n-butanol/acetic acid/water. First plate is TLC plate 1. Second plate is TLC plate 2.

What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a TLC lab and how can that hypothesis be tested?

What are the elution techniques usually followed in HPLC?

How is TLC useful in determining the identity of a sample?

Organic Chemistry, Column questions