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Complete the following tasks. You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you to study mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from different samples through PCR. You will need to run a total of 7 PCRS: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix-all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of these components, and the needed working concentrations for the PCR itself. Complete the table by supplying the needed volumes of each component for a single reaction and for the master mix (the first row has been filled in as an example). Stock Working Volume for 1 Volume for 7 PCR Component concentration concentration reaction (uL) reactions (uL) Buffer for Taq 10X 1X 1.0 7.0 polymerase Forward primer 40 11M 101M

Complete the following tasks. You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you to study mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from different samples through PCR. You will need to run a total of 7 PCRS: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix-all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of these components, and the needed working concentrations for the PCR itself. Complete the table by supplying the needed volumes of each component for a single reaction and for the master mix (the first row has been filled in as an example). Stock Working Volume for 1 Volume for 7 PCR Component concentration concentration reaction (uL) reactions (uL) Buffer for Taq 10X 1X 1.0 7.0 polymerase Forward primer 40 11M 101M

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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AutoSave O Off # 2 Mol Bio Mutation QUESTIONS - Saved to this Pc - O Search A MST MCHUNU MT File Home Insert Design Layout References Mailings Review View Help Chemistry 合 Share P Comments (i) SIGN IN TO OFFICE It looks like your stored credentials are out of date. Please sign in as 20******@ke****** za so we can verify your subscription. Sign In Complete the following tasks. You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you to study mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from CDNA extracted from different samples through PCR. You will need to run a total of 7 PCRS: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix-all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes, The table below lists the different components for PCR, the available stock concentrations of these components, and the needed working concentrations for the PCR itself. Complete the table by supplying the needed volumes of each component for a single reaction and for the master mix (the first row has been filled in as an example). Stock Working Volume for 1 Volume for 7 PCR Component concentration concentration reaction (uL) reactions (uL) Buffer for Taq 10X 1X 1.0 7.0 polymerase Forward nrimer 40 11M 10 11M Page 1 of 3 445 words English (United States) D Focus 100% ENG 17:34 O Type here to search 22°C INTL 2022/01/29
Transcribed Image Text:AutoSave O Off # 2 Mol Bio Mutation QUESTIONS - Saved to this Pc - O Search A MST MCHUNU MT File Home Insert Design Layout References Mailings Review View Help Chemistry 合 Share P Comments (i) SIGN IN TO OFFICE It looks like your stored credentials are out of date. Please sign in as 20******@ke****** za so we can verify your subscription. Sign In Complete the following tasks. You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you to study mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from CDNA extracted from different samples through PCR. You will need to run a total of 7 PCRS: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix-all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes, The table below lists the different components for PCR, the available stock concentrations of these components, and the needed working concentrations for the PCR itself. Complete the table by supplying the needed volumes of each component for a single reaction and for the master mix (the first row has been filled in as an example). Stock Working Volume for 1 Volume for 7 PCR Component concentration concentration reaction (uL) reactions (uL) Buffer for Taq 10X 1X 1.0 7.0 polymerase Forward nrimer 40 11M 10 11M Page 1 of 3 445 words English (United States) D Focus 100% ENG 17:34 O Type here to search 22°C INTL 2022/01/29
AutoSave O Off # 2 Mol Bio Mutation QUESTIONS - Saved to this Pc - Search A MS T MCHUNU MT File Home Insert Design Layout References Mailings Review View Help Chemistry 合 Share P Comments (i) SIGN IN TO OFFICE It looks like your stored credentials are out of date. Please sign in as 20******@ke****** za so we can verify your subscription. Sign In Stock Working Volume for 1 Volume for 7 PCR Component concentration concentration reaction (uL) reactions (uL) Buffer for Taq| 10X 1X 1.0 7.0 polymerase Forward primer 40 uM 1.0 uM Reverse primer 10 uM 1.0 uM ANTPS 25 mM 1 mM Taq polymerase 200 U/ul 1 U/ul Sterile distilled (to fill reaction n/a deionized water volume to 10 ul) Total 9.0 ul 63.0 ul 1.0 (added separately to each reaction Template 400 ng/ul 40 ng/ul after master mix is distributed to different tubes) Total 10.0 70.0 Page 1 of 3 English (United States) O Focus 445 words 100% ENG 17:34 O Type here to search 22°C INTL 2022/01/29
Transcribed Image Text:AutoSave O Off # 2 Mol Bio Mutation QUESTIONS - Saved to this Pc - Search A MS T MCHUNU MT File Home Insert Design Layout References Mailings Review View Help Chemistry 合 Share P Comments (i) SIGN IN TO OFFICE It looks like your stored credentials are out of date. Please sign in as 20******@ke****** za so we can verify your subscription. Sign In Stock Working Volume for 1 Volume for 7 PCR Component concentration concentration reaction (uL) reactions (uL) Buffer for Taq| 10X 1X 1.0 7.0 polymerase Forward primer 40 uM 1.0 uM Reverse primer 10 uM 1.0 uM ANTPS 25 mM 1 mM Taq polymerase 200 U/ul 1 U/ul Sterile distilled (to fill reaction n/a deionized water volume to 10 ul) Total 9.0 ul 63.0 ul 1.0 (added separately to each reaction Template 400 ng/ul 40 ng/ul after master mix is distributed to different tubes) Total 10.0 70.0 Page 1 of 3 English (United States) O Focus 445 words 100% ENG 17:34 O Type here to search 22°C INTL 2022/01/29
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