Compared to a protein with a low Km, a protein with a high Km possess ________ for the substrate. Select one: a. lower Vmax b. higher affinity c. lower affinity d. none of the above e. higher Vmax
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Enzymes are proteins and are basically catalyst that increases the rate of reaction. km is the characteristics of an enzyme and it is measured by enzyme affinity for the substrate.
There are a few important points that should be kept in mind.
- Km is Michaelis constant, the concentration of substrate at which velocity reaches half its maximum velocity.
- Lower value of km describes the greater affinity of the enzymes for the substrate.
- Km inversely is proportional to enzyme affinity of the substrate.
Michaelis-Menten studied the relationship between reaction velocity and substrate concentration. According to him when the rate of an enzyme-catalyzed reaction is measured at a varying substrate concentration, the rate depends on substrate concentration.
V=Vmax [S] / Km+[S]
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