Christ purified LDH and stopped at 65% pellet fraction. He finished data collection as listed in the table below, took off for the weekend early. Please finish the table for him by filing out the blanks. Crude Chris's 65% pellet Total protein concentration in mg/ml U/ml 20 100 300 Total volume in ml 50 12 Total U blank 1 blank 2 Specific Activity blank 3 blank 4 Fold Purification blank 5 blank 6
Christ purified LDH and stopped at 65% pellet fraction. He finished data collection as listed in the table below, took off for the weekend early. Please finish the table for him by filing out the blanks. Crude Chris's 65% pellet Total protein concentration in mg/ml U/ml 20 100 300 Total volume in ml 50 12 Total U blank 1 blank 2 Specific Activity blank 3 blank 4 Fold Purification blank 5 blank 6
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Help?!
Transcribed Image Text:**LDH Purification Data Analysis**
Christ purified LDH (Lactate Dehydrogenase) and stopped at the 65% pellet fraction. He completed data collection as shown in the table below and left for the weekend early. You are tasked with completing the table by filling in the blanks.
### Table of Purification Data
| | Crude | Chris's 65% Pellet |
|------------------------------|-------|--------------------|
| Total protein concentration in mg/ml | 20 | 5 |
| U/ml | 100 | 300 |
| Total volume in ml | 50 | 12 |
| **Total U (Units)** | blank 1 | blank 2 |
| **Specific Activity** | blank 3 | blank 4 |
| **Fold Purification** | blank 5 | blank 6 |
### Blanks to Fill
- **Blank 1:**
- **Blank 2:**
- **Blank 3:**
- **Blank 4:**
- **Blank 5:**
- **Blank 6:**
To fill in the blanks:
1. **Calculate Total U (Units):** Multiply U/ml by Total volume in ml.
2. **Calculate Specific Activity:** Divide Total U by Total protein concentration in mg/ml.
3. **Calculate Fold Purification:** Divide Specific Activity of Chris's 65% pellet by Specific Activity of Crude.
Please complete the calculations and fill in the blanks accordingly.
Expert Solution
Step 1
The study of rate of enzyme catalyzed biological reactions is known as enzyme kinetics. In enzyme kinetics, the rate of reaction is calculated under normal conditions and under the varying conditions. Enzyme is a protein that gets bind to the substrate and allow the substrate to get converted into the product.
Step by step
Solved in 5 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON