Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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**LDH Purification Data Analysis** Christ purified LDH (Lactate Dehydrogenase) and stopped at the 65% pellet fraction. He completed data collection as shown in the table below and left for the weekend early. You are tasked with completing the table by filling in the blanks. ### Table of Purification Data | | Crude | Chris's 65% Pellet | |------------------------------|-------|--------------------| | Total protein concentration in mg/ml | 20 | 5 | | U/ml | 100 | 300 | | Total volume in ml | 50 | 12 | | **Total U (Units)** | blank 1 | blank 2 | | **Specific Activity** | blank 3 | blank 4 | | **Fold Purification** | blank 5 | blank 6 | ### Blanks to Fill - **Blank 1:** - **Blank 2:** - **Blank 3:** - **Blank 4:** - **Blank 5:** - **Blank 6:** To fill in the blanks: 1. **Calculate Total U (Units):** Multiply U/ml by Total volume in ml. 2. **Calculate Specific Activity:** Divide Total U by Total protein concentration in mg/ml. 3. **Calculate Fold Purification:** Divide Specific Activity of Chris's 65% pellet by Specific Activity of Crude. Please complete the calculations and fill in the blanks accordingly.
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Transcribed Image Text:**LDH Purification Data Analysis** Christ purified LDH (Lactate Dehydrogenase) and stopped at the 65% pellet fraction. He completed data collection as shown in the table below and left for the weekend early. You are tasked with completing the table by filling in the blanks. ### Table of Purification Data | | Crude | Chris's 65% Pellet | |------------------------------|-------|--------------------| | Total protein concentration in mg/ml | 20 | 5 | | U/ml | 100 | 300 | | Total volume in ml | 50 | 12 | | **Total U (Units)** | blank 1 | blank 2 | | **Specific Activity** | blank 3 | blank 4 | | **Fold Purification** | blank 5 | blank 6 | ### Blanks to Fill - **Blank 1:** - **Blank 2:** - **Blank 3:** - **Blank 4:** - **Blank 5:** - **Blank 6:** To fill in the blanks: 1. **Calculate Total U (Units):** Multiply U/ml by Total volume in ml. 2. **Calculate Specific Activity:** Divide Total U by Total protein concentration in mg/ml. 3. **Calculate Fold Purification:** Divide Specific Activity of Chris's 65% pellet by Specific Activity of Crude. Please complete the calculations and fill in the blanks accordingly.
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The study of rate of enzyme catalyzed biological reactions is known as enzyme kinetics. In enzyme kinetics, the rate of reaction is calculated under normal conditions and under the varying conditions. Enzyme is a protein that gets bind to the substrate and allow the substrate to get converted into the product.

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