Choose the correct option. Which one of following techniques is used as fractionation as well as for determination of molecular weight determination. a) Gel Permeation Chromatography c) End group analysis c) Vapor pressure osmometry d) Fractional Precipitation technique
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Choose the correct option.
Which one of following techniques is used as fractionation as well as for determination of molecular weight determination.
a) Gel Permeation Chromatography
c) End group analysis
c) Vapor pressure osmometry
d) Fractional Precipitation technique
Step by step
Solved in 2 steps
- Explain the following terms used in chromatography i) Dead time ii) Number of theoretical plates Describe the sample properties that would be suitable for; i) Gas chromatography (GC) analysis ii) High-performance liquid chromatography (HPLC) analysisIn chromatography, the compound that is least soluble in the mobile phase a) Travels the furthest away from the line of origin b) Stays closest to the line of originSeparation of Components from Mixture of Red and Blue Inks by Paper Chromatography Give an explanation about this table result
- What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography (TLC)? Please shortly answer at your own words. Answer should be to the point ( specific 4-5 lines).In have narrow peaks are said to chromatography, a) a large plate height (H) b) a small plate height (H) c) a small number of theoretical plates (N)Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (i) (ii) (iii) (iv) 0.01 TO 0.8 1.2 0". BEO - 2.0 1757 $122. 2.8 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?
- A method of quantitative determination of the concentration of constituentsof a sample analyzed by gas chromatography is area normalization. Thiscomplete elution of all sample constituents is required. Thearea of each peak is measured and corrected for the detector response to the diffe- renteseluatos. This correction involves dividing the area by an empiricalcorrection factorDetermined. The concen ration of the analyte is found from the ratio between itscorrected and the total corrected area of all peaks. For a chromatogram containing threerelative areas were determined as 16.4; 45,2; and 30.2, in the order ofincreased retention time. Calculate the percentage of each compound if therelative responses from the detector are 0.65, 0.73 and 0.85, respectively.Fill in the blanks: a) The component of the mixture that becomes a water soluble salt by treating the two component mixture with sodium hydroxide solution has the highest _______________. b) The lower phase in equipment used for liquid-liquid extraction in a water-dichloromethane mixture is_____________, because _______________. c) The component of the mixture that becomes a water-soluble salt is the one with the greatest ______________Is longitudinal diffusion of the solute a more serious problem in gas or in liquid chromatography? gas chromatography O liquid chromatography Why is longitudinal diffusion a more serious problem in this type of chromatography? Gas chromatography is carried out at higher temperatures than liquid chromatography. The solute must be volatile for application of gas chromatography. The linear velocity in gas chromatography is greater than the linaer velocity in liquid chromatography. Diffusion coefficients of gases are much greater than those of liquids.
- In which mode of chromatography do solute and solvent molecules migrate in and out the stationary phase based on the solute’s equilibrium between the stationary and mobile phases. a) adsorption b) partition c) extraction d) absorbanceThe solvent in a collodial system is also known as the [ ?] phase. A dispersed particle dispersion mediumCan paper chromatography be used to separate and identify very volatile substances? How do I prove this.