Chloroplast ATP synthase contains 14 c subunits. How many protons must be translocated to the thylakoid lumen to support the synthesis of one ATP? Please help.
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10. Chloroplast ATP synthase contains 14 c subunits. How many protons
must be translocated to the thylakoid lumen to support the synthesis
of one ATP? Please help.
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- The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)Chloroplast ATP synthase contains 14 c subunits. How many protons must be translocated to the thylakoid lumen to support the synthesis of one ATP?Protons move across the thylakoid membrane in both directions during the light reactions of photosynthesis...... 1. When protons move in to the thylakoid, are they moving up or down their concentration gradient? (From low to high concentration or from high to low concentration?) 2. Since protons are charged, they cannot freely diffuse across a plasma membrane. What protein(s) facilitate the transport of protons in to the thylakoid?
- Identify the chemical basis for ApH and AY across the chloroplast thylakoid membrane by dragging the descriptions to their targets. Be sure to notice that the upper arrow iindicates ApH and the lower arrow indicates ΔΨ. ATP synthase complex H+ N ADP + P₁ Light energy ATP H*N Photosystem I/II- Chloroplast N side Aus PN ApH T + Thylakoid membrane HTp H+p Lumen Stroma P side Proton circuit A B High H concentration Low positive charge High positive charge Low H+ concentration Within the image, identify the types of proton translocation by dragging each label to its target. O XH₂ 2H+ + Z 2 H* ZH₂ O XH₂ Z 2H+ ZH₂ 2H+ C A B Proton pump Redox loopWhen researchers shifted isolated chloroplasts from a low pH solution to a more alkaline (higher pH) solution, ATP synthesis occurred even in the absence of light! This was an experiment used to support the chemiosmosis mechanism of ATP formation in chloroplasts. Why?Transport across the inner membrane of chloroplasts depends on the proton gradient established during ATP synthesis, similar to mitochondria. Can someone explain why this statement is false?
- Diagram 4: The Light Reactions of Photosynthesis STROMA dow concentralion Photonystem B Cytch e Photosystem Light omplex Light NADP reductoe NADP NADPH THYLAKOID SPACE igh ooncentration) Cavin Cyle Thylakid menbrane ATP STROMA ow H eone sythase ADP ATP Diagram 4 shows a single thylakoid membrane taken from a chloroplast inside of a leaf cell. The events of the light dependent cycle that take place along the thylakoid membrane are shown. Light energy is captured by O ATP synthase O Photosystems I and II O NADP+ reductase The thylakoid membrane's lipid structures DroviousFor the following questions, choose one to discuss: chloroplast/photosynthesis State at the outset which one you will discuss. A) What role do proton gradients play in the process of photosynthesis proton gradients allow B) Where are proton gradients formed? Within what structures are they seen in chloroplasts How do the structures help them to maintain a gradient? C) Explain where and how the chloroplast or mitochondria uses passive transport and active transport to complete photosynthesis or cellular respiration.Thylakoids were isolated from chloroplasts and incubated in the dark in an acidic solution (pH 4) to equilibrate the pH. After 30 minutes, the thylakoids were transferred to a basic solution (pH 8) and kept in the dark. Will this system produce ATP? Explain. Will this system produce G3P? Explain.
- Could a plant cell produce ATP through chemiosmosis if the thylakoid membranes of its chloroplasts were “leaky” with regard to protons? Explain.In chloroplasts, protons are pumped out of thestroma across the thylakoid membrane, whereas in mito-chondria, they are pumped out of the matrix across thecrista membrane. Explain how this arrangement allowschloroplasts to generate a larger proton gradient acrossthe thylakoid membrane than mitochondria can generateacross the inner membrane.For the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the protons from the photosystems are transported, like with the mitochondrial inner membrane, against the gradient from the lumen to the stroma. However, the pH(lumen) – pH (stroma) is much larger, about 3.4. Calculate delta psi (the electric potential) for the thylakoid membrane, given that ∆G of proton transport is about the same as for the mitochondrion. Then comment on its permeability to ions, compared to the mitochondrial inner membrane’s permeability to ions.