Homework Help is Here – Start Your Trial Now!
Science
Biology
CDNAS and ESTS reveal exons or gene ends in genome searches Exon 2 Exon 1 Exon 3 Exon 4 Genomic DNA 240 241 528 529 696 697 816 3 CDNA 5' EST 3' EST ESTS FIGURE 14-10 Alignment of fully sequenced complementary DNAS (CDNAS) and expressed sequence tags (ESTS) with genomic DNA. The dashed lines indicate regions of alignment; for the CDNA, these regions are the exons of the gene. The dots between segments of CDNA or ESTS indicate regions in the genomic DNA that do not align with CDNA or EST sequences; these regions are the locations of the introns. The numbers above the CDNA line indicate the base coordinates of the CDNA sequence, where base 1 is the 5'-most base and base 816 is the 3'-most base of the CDNA. For the ESTS, only a short sequence read is obtained from each end (5' and 3') of the corresponding CDNA. These sequence reads establish the boundaries of the transcription unit, but they are not informative about the internal structure of the transcript unless the EST sequences cross an intron (as is true for the 3' EST depicted here).

CDNAS and ESTS reveal exons or gene ends in genome searches Exon 2 Exon 1 Exon 3 Exon 4 Genomic DNA 240 241 528 529 696 697 816 3 CDNA 5' EST 3' EST ESTS FIGURE 14-10 Alignment of fully sequenced complementary DNAS (CDNAS) and expressed sequence tags (ESTS) with genomic DNA. The dashed lines indicate regions of alignment; for the CDNA, these regions are the exons of the gene. The dots between segments of CDNA or ESTS indicate regions in the genomic DNA that do not align with CDNA or EST sequences; these regions are the locations of the introns. The numbers above the CDNA line indicate the base coordinates of the CDNA sequence, where base 1 is the 5'-most base and base 816 is the 3'-most base of the CDNA. For the ESTS, only a short sequence read is obtained from each end (5' and 3') of the corresponding CDNA. These sequence reads establish the boundaries of the transcription unit, but they are not informative about the internal structure of the transcript unless the EST sequences cross an intron (as is true for the 3' EST depicted here).

Biochemistry
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

In Figure 14-10, expressed sequence tags (ESTs) are
aligned with genomic sequence. How are ESTs helpful in
genome annotation?

CDNAS and ESTS reveal exons or gene ends in genome searches Exon 2 Exon 1 Exon 3 Exon 4 Genomic DNA 240 241 528 529 696 697 816 3 CDNA 5' EST 3' EST ESTS FIGURE 14-10 Alignment of fully sequenced complementary DNAS (CDNAS) and expressed sequence tags (ESTS) with genomic DNA. The dashed lines indicate regions of alignment; for the CDNA, these regions are the exons of the gene. The dots between segments of CDNA or ESTS indicate regions in the genomic DNA that do not align with CDNA or EST sequences; these regions are the locations of the introns. The numbers above the CDNA line indicate the base coordinates of the CDNA sequence, where base 1 is the 5'-most base and base 816 is the 3'-most base of the CDNA. For the ESTS, only a short sequence read is obtained from each end (5' and 3') of the corresponding CDNA. These sequence reads establish the boundaries of the transcription unit, but they are not informative about the internal structure of the transcript unless the EST sequences cross an intron (as is true for the 3' EST depicted here).
Transcribed Image Text:CDNAS and ESTS reveal exons or gene ends in genome searches Exon 2 Exon 1 Exon 3 Exon 4 Genomic DNA 240 241 528 529 696 697 816 3 CDNA 5' EST 3' EST ESTS FIGURE 14-10 Alignment of fully sequenced complementary DNAS (CDNAS) and expressed sequence tags (ESTS) with genomic DNA. The dashed lines indicate regions of alignment; for the CDNA, these regions are the exons of the gene. The dots between segments of CDNA or ESTS indicate regions in the genomic DNA that do not align with CDNA or EST sequences; these regions are the locations of the introns. The numbers above the CDNA line indicate the base coordinates of the CDNA sequence, where base 1 is the 5'-most base and base 816 is the 3'-most base of the CDNA. For the ESTS, only a short sequence read is obtained from each end (5' and 3') of the corresponding CDNA. These sequence reads establish the boundaries of the transcription unit, but they are not informative about the internal structure of the transcript unless the EST sequences cross an intron (as is true for the 3' EST depicted here).
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genome annotation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Concepts of Biology
Concepts of Biology
Biology
ISBN:
9781938168116
Author:
Samantha Fowler, Rebecca Roush, James Wise
Publisher:
OpenStax College
SEE MORE TEXTBOOKS