A. If a student performs a Gram stain on a Gram negative bacterium and stops the stain technique after the iodine step and rinse, what color would you predict the cells to be if you looked at it right now under the microscope?

You use gram staining procedure to stain a bacterium that lacks a cell wall. What color will the bacterium be after staining procedure is finished?

microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?

After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.

Observation: What color would the bacterial cells have when viewed theoretically under the microscope after the following treatment? Gram Ammonium oxalate Gram's iodine Ethyl Alcohol Safranin reaction Crystal violet Gram positive Gram negative

Which part of the microscope is used to adjust the light? (pick one) i. h. a. d. c.

Gram Stain Gram Reaction (Gram- positive or Gram- negative): Cellular morphology (shape & arrangement): _________________________________

Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella Stain

What type of stain is the Gram stain and what does it rely on for meaningful results? Elaborate your answer.

Examine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information: A. Shape....... gram stain( +) , Gram (-) or both B. Stained color under microscope C. PG Wall thick, thin or both