Can xylane powder from beechwood be dissolved in M9 media? I'm evaluating the production of hydrogen with E. coli (it has a construction that alows this strain to depolimerize xylane into xylose monomers by an endoxylanase), but i have a limited amount of this xylane, and i read TB, AM1 & M9 mediums were used, but they don't say if they just poured the xylan powder into the media solution, i also read they autoclaved it with a soft acid solution and then neutralized it with a basic soluction, but this is an acid hydrolyzate (AH) plus enzymatic hydrolysis (EH). It ´s certain that using both would enhance available xylose concentration, but i want to evaluate EH by itself, and i still can't find if it is just added as powder to the media. Would it dissolve? do i have to add some kind of buffer to the media?.

I'm evaluating the production of hydrogen with E. coli (it has a construction that alows this strain to depolimerize xylane into xylose monomers by an endoxylanase), but i have a limited amount of this xylane, and i read TB, AM1 & M9 mediums were used, but they don't say if they just poured the xylan powder into the media solution, i also read they autoclaved it with a soft acid solution and then neutralized it with a basic soluction, but this is an acid hydrolyzate (AH) plus enzymatic hydrolysis (EH). It ´s certain that using both would enhance available xylose concentration, but i want to evaluate EH by itself, and i still can't find if it is just added as powder to the media. Would it dissolve? do i have to add some kind of buffer to the media?.