Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Calculate the percentage efficiency of extraction of phycocyanin in sample y, if the amount of phycocyanin is 57.8 mg, and the starting amount in sample x was 118.1 mg. Your answer should have one decimal place.
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- Most of the following conclusions regarding the photo shown above are correct Except: All of the following are correct regarding the disk diffusion test photos EXCEPT : Zone of inhibition Chlorine Chlorine Chlorine O-phenylphenol Hexachlorophene O-phenylphenol Hexachlorophene Hexachlorophene O-phenylpheno Quat Quat Quat Staphylococcus aureus (gram-positive) Escherichia coli Pseudomonas aeruginosa (gram-negative) (gram-negative) Select one: O to. Gram negative bacteria are the most sensitive to chemical agents O b. Only one of the four chemicals affected Pseudomonas O c. Gram positive bacteria are the most sensitive to disinfectants O d. Hexachlorophene was effective only against Gram positive bacteria. O and. Chlorine was effective against all bacteria bacteria testedarrow_forwardWhat if all the data above was the same EXCEPT with the change of G & X? Their new data is below. Would base X still be T? G & X [G] = 0.0447315M [X] = 0.0447315M [GX] = 0.0026845M 1.3416arrow_forwardCalculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.arrow_forward
- What does an A280 of 0.000 indicate? What does this reading indicate in the context of your column chromatography? Are there still proteins adhering to the column? Do you know anything about the proteins which are still adhering to the column?arrow_forwardAspergillus can be used to produce α-amylase (α-AM), which is an enzyme that hydrolyzes the glycosidic bonds in starch molecules into maltose (disaccharide). Pure α-AM has a specific activity of 56.00 U/mg. The α-AM is secreted into the culture media during production (after 5 days, production is complete). Following, the Aspergilluscells are separated by centrifugation and discarded. The culture supernatant (with α-AM) is filtered giving a clarified supernatant with a volume of 100.0 L, activity of 3.00 U/mL, and total protein concentration of 0.650 mg/mL. After purification, the product stream (7.50 L) contains α-AM (25.00 U/mL) and a total of 6.788 x 103 mg total protein. Calculate the following: Concentration of total protein (g/L) and total units of activity in the clarified supernatant and purification product stream. Specific activities (U/g) of the clarified supernatant and purification product stream. Consider significant figures in your answers (may want to use scientific…arrow_forward
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