Calculate the concentration of lycopene in your orange fraction using using Beer's law equation A=ExBxC A is the measured absorbance, E is the molar extinction coefficient (M-1cm-1) at a particular wavelength, B is the path length (in cm) and C is the analyte concentration (M).  A= 0.602. Wavelenght is 500.95nm.  Molar extinction coefficients is 123,000M-1cm-1

SPECTROPHOTOMETRY Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R? value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be…

permanganate solution using the color wheel. ART II: INVESTIGATING ABSORBANCE AND PATHLENGTH 12. Based on Beer's law (A = ɛlC, A = absorbance, ɛ = molar absorptivity, I pathlength and C = concentration), what do you predict is the relationship between pathlength and absorbance? What about pathlength and transmittance?

The transmitance is 14.4%

A student mixed 5.00mL of 0.0020M Fe(NO3)3, 3.00mL of 0.0020M KSCN, and 3.00mL of distilled water. The mixture reacted to form a reddish solution indicating that FeSCN2+ was formed, according to the equation below. An absorbance of 0.21 was measured for this solution in a Spectrophotometer set at a wavelength of 447nm. Fe³+ (aq) + SCN (aq) FeSCN2+ (aq) A calibration curve was used to find the equilibrium concentration of the product of this reaction: y = 1895x + 0.027 Calculate the equilibrium concentration of the Fe³+ Report your answer to 4 sig figs

A compound with a molecular weight of 229.61 g/mol was dissolved in 50.0 mL of water. 1.00 mL of this solution was placed in a 10.0 mL flask and diluted to the mark. The absorbance of this diluted solution at 510 nm was 0.472 in a 1.000 cm cuvet. The molar absorptivity of the compound, at 510 nm, is 6,310 M-1 cm-1. Calculate the concentration of the compound in the initial 50.0 mL solution. A. 1.50 x 10-5 M B. 7.48 x 10-5 M C. 7.48 x 10-4 M D. 7.48 x 10-6 M

A calibration curve is made for the absorbance as a function of concentration of blue dye (in mg/L).  The slope of the curve is 0.131; the y-intercept is -0.01869; the correlation is 0.993.  If a consumer sample has an absorbance of 0.120 on the same instrument, what is its concentration of blue dye, in mg/L? a. 0.034 b. 0.935 c. 0.993 d. 1.06

Maria was tasked to determine the concentration of a Fe2(SO4)3 solution. She prepared 5 different standards of Fe2(SO4)3 using the table below as her guide. She ran the standards and the unknown solution through UV-VIS spectroscopy and recorded the absorbances of each solution.   HINT: use the dilution equation to determine the concentration of Fe2(SO4)3 in each test tube before preparing the standard curve. NOTE: No need to force the line to pass through zero. Just graph the data as is.   What is the slope of the line of the standard curve from the given data? What is the concentration of the unknown solution?

An unknown amount of a compound with a molecular mass of 270.57 g/mol is dissolved in a 10 mL volumetric flask. A 1.00 mL aliquot of this solution is transferred to a 25 mL volumetric flask, and enough water is added to dilute to the mark. The absorbance of this diluted solution at 355 nm is 0.495 in a 1.000 cm cuvette. The molar absorptivity for this compound at 355 nm is €355 = 6149 M-¹cm-¹. What is the concentration of the compound in the cuvette? concentration: What is the concentration of the compound in the 10 ml flask? concentration: How many milligrams of compound were used to make the 10 mL solution? mass: M M mg