Calculate the amount of ATP’s that will be formed during the oxidative phosphorylation process if: 46 NADH + H and 12 FADH + H is available
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Calculate the amount of ATP’s that will be formed during the oxidative phosphorylation process if: 46 NADH + H and 12 FADH + H is available .
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- Assuming all the NADH+H+ and FADH2 are used for oxidative phosphorylation, how many net ATP are made from 1 molecule of glucose? (hint: don’t forget to also include substrate level phosphorylation)Assuming that the glycerol 3-phosphate shuttle is operative, a complete oxidation of three molecules of glucose (by a eukaryotic cell) into carbon dioxide and water would produce approximately ATP molecules via oxidative phosphorylation only. (Consider NADH = 2.5 ATP and FADH2 = 1.5 ATP)In the presence of excess oxygen, a complete oxidation of seven molecules of glucose into carbon dioxide and water, by a yeast cell, would produce approximately ATP molecules via oxidative phosphorylation only. (Consider NADH = 3 ATP and FADH2 = 2 ATP)
- If decanoic acid (10 : 0) undergoes complete β-oxidation in mitochondria, how many net ATP is yielded? (NADH = 2.5 ATP; FADH2 = 1.5 ATP; Acetyl CoA = 10 ATP) Answer in number onlyHow many protons are required to synthesize one ATP by F1F0-ATPase containing (a) 10 or (b) 15 c subunits?Calculate the total ATP produced in the catabolism of glucose. Follow the table provided below. If NADH = 3 ATP, If NADH = 2.5 АТР, FADH, 1.5 FADH, = 2 ATP ATP Glycolysis Pyruvate shuttle Krebs cycle ETC TOTAL If NADH = 3 ATP, If NADH = 2.5 %3D FADH2 = 2 ATP ATP, FADH2 = 1.5 ATP Alcohol Fermentation Lactic acid fermentation
- A new ATP-producing protein is discovered that couples ATP production to the oxidation of NADPH by oxidative phosphorylation. Assume that the value of ΔGo for ATP synthesis is 30 kJ•mol−1. If this protein only produces 1 molecule of ATP per reaction that consumes one NADPH: a. How much free energy is wasted, under standard conditions?b. How many more ATP molecules could be created by a perfectly efficient electron transport chain from one NADPH?Give all the reactions that will produce ATP either by substrate-level phosphorylation (SLP) or by oxidative phosphorylation (OP). If the given require a shuttle system, please indicate both MA shuttle and GP shuttle and give the ATP produced. Given: glucose 6-phosphate to 2succinly CoAThe glutamate dehydrogenase (GDH) catalyses the following reaction: H +H₂N- -C -COO CH₂ CH₂ COO™ acide glutamique + NAD + H₂O GDH Time (min) A340 COO™ c=o CH₂ 5 1 2 3 4 1.760 1.718 1.675 1.635 1.595 CH₂ COO + NH4+ NADH + H+ acide a-cétoglutarique The activity of GDH is monitored in the sense of the formation of glutamate using the following conditions: 0.2 mL of 5 M ammonium sulphate 2.4 mL of buffer at pH 8 0.1 mL of NADH at 6.15 mg.mL-¹ (M = 709 g.mol-¹) 0.2 mL of 1 M a-ketoglutarate solution Warm mixture at 25 °C for 5 min Add 0.1 mL of GDH solution containing 1.6 mg.mL-¹protein to start the reaction. The change in absorbance at 340 nm is monitored, in a 1-cm cuvette, every minute for 10 min. Results are given in the table below: Data ENADH at 340 nm = 6220 M¹.cm7 6 7 8 9 10 1.550 1.510 1.489 1.476 1.451 Calculate ammonium sulphate, NADH, concentrations in the reaction medium at t = 0. proteins - Draw the graph A = f(t). Calculate A340 at t = 0 and place this point on the curve. -…
- Give all the reactions that will produce ATP either by substrate-level phosphorylation (SLP) or by oxidative phosphorylation (OP). If the given require a shuttle system, please indicate both MA shuttle and GP shuttle and give the ATP produced. Given: fructose 6-phosphate to 2pyruvateTell how many ATPs result from from oxidative phosphorylation of one NADH; tell how many ATPs result from oxidation phosphorylation of one FADH2.Describe the process of oxidative phosphorylation. In your description, include the terms NADH, FADH2, ATP, mitochondria (or mitochondrial), glycolysis, citric acid cycle, electron (or electrons), proton (or protons), and oxygen. Be sure to describe how ATP molecules get produced, and how many ATP molecules are produced per FADH2 and NADH (note; NADH from outside the mitochondria generate a different number of ATP compared to NADH inside the mitochondria; your description should indicate both quantities for full credit.) vas 19llo ton lle vowor 1esb