Malate dehydrogenase is an enzyme that converts malate to oxaloacetate in the last stage of the TCA Cycle (citric acid cycle/Krebs cydle), represented by the following equation: NAD+ Malate Oxaloacetate NADH A group of students carried out an experiment to determine the optimum temperature for the activity of a commercially produced malate dehydrogenase (from yeast) at several different temperatures. A series of test tubes was set up containing 2.0 cm of phosphate buffer with a pH of 7.5, 0.1 cm? of NADH, 0.1 cm3 of malate dehydrogenase and 0.7 cm3 of water. The tubes were incubated in water baths at the various temperatures for 5 minutes. At certain time intervals each tube was placed in a colorimeter, set at an absorbance value of 1.0. The reaction was then started by adding 0.1 cm? of oxaloacetic acid to the tube. Enzyme activity was measured by following the decrease in absorbance for 120 seconds. The experiment was repeated to give duplicate results. Enzyme activity was then calculated as pmoles per mg of (enzyme) protein. The results are shown in Table 1 below: Table 1: Results to show the activity of malate dehydrogenase at various temperatures. Enzyme activity (umoles per mg protein) Temp. (°C) Average (to two decimal points 0.20 12.90 Test 2 (Duplicate test) Test 1 0.20 0.20 12.7 0.00 10.0 13.0 20.0 30.0 40.0 52.0 91.3 82.7 50.2 90.0 79.3 51.10 90.70 81.00 40.80 16.60 50.0 41.5 40.0 60.0 70.0 17.2 16.0 9.90 10.0 9.95 a) On the graph paper provided, plot the data in a suitable form. b) Briefly explain the effect of temperature on malate dehydrogenase activity. What is the optimum temperature for the activity of malate dehydrogenase (calculated from your graph)? c) d) The value you have calculated in (c) above may not be the true optimum temperature for the enzyme. Describe how you could modify the experiment to get a more accurate optimum value. Why were the tubes incubated for 5 minutes prior to the addition of oxaloacetic acid to start the reaction? e)

temporalis- -3 8. -4 A. mentalis 2 B. sternocleidoastoid 13 C. buccinator D. platysma E. masseter F. orbicularis oris G.orbicularis oculi H. zygomaticus 1 trapezius J. frontalis (or occipitofrontalis, to be precise)

The highlighted vessel has 3 branches. Name one of them. From LT VA16 Life-size Muscle Torso 27 part, 3B Scientific

Please Answer all the questions don't need to explain everything...   1. Intervertebral discs contribute to the painful condition of disc herniation.  Which option below correctly describes the anatomy of this condition?   A. The nucleus pulposus weakens and the gel-like annulus fibrosis protrudes causing the herniation B. The annulus fibrosis weakens and the gel-like nucleus pulposus protrudes causing the herniation C. None of the above - as we age the annulus fibrosis gets stronger  D. The annulus fibrosis weakens and the entire posterior portions of the fibrosis fold causing pain.     1.c The sternal angle is formed from which bones listed below:1. Manubrium2. Body of sternum3. Xiphoid process   A. 1 and 3 B. 1 and 2 C. None of the above - the sternal angle is not an anatomical structure D. 2 and 3   1d.  Which part of the rib forms an articulation (joint) with the body of thoracic vertebra?   A. costal cartilage B. head of the rib C. neck of the rib D. tubercle of the rib

Please guide me in answering the wuestions below!