The bacterium Dontuplodemeus tocheggus has a generation time of 20 minutes. Starting with one cell in 500 mL of culture medium, how many minutes would it take to reach a cell density of approximately one million cells per mL? Assume all cells remain viable. a. 330 b. 660 c. 380 d. 400 e. 580

Two bacterial species have generation times of 20 min and 18 hours respectively. From the below list, choose all true statements. A. The 2nd species must not be at the optimal temperature for its growth B. different species may have very different generation times  C. optimal culture conditions may not have been used for one or both species D. stationary phase will be reached much faster with the first species

Which isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count?  A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply.  ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms?  A. inoculum B. culture medium C. diluent D. microbial culture

Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to 4.6x108 CFU/mL in 3 hours. Be sure to use units in your answer.