Bacteria are often the preferred hosts for genetic engineering projects by splicing in novel genes from eukaryotes into plasmids, which are moved into competent bacteria. For instance, the gene for human insulin was isolated and moved into a bacterium, which can now produce the much-needed chemical. Previously, type 1 diabetics had to rely on professionals that gathered insulin from human cadavers, cows, and pigs. In order for this feat of genetic engineering to occur, researchers had to start with an unspliced mRNA transcript for h insulin. Agree/Disagree? Explain your response.

Q: A lac operon containing one mutation was cloned into a plasmid, which was introduced by…

A: the lac operon system present in bacteria

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: The lac operon is an example of ________ control in which the presence of an ________ is required…

A: lac operon is present in E.coli and it produce the enzymes which are required for the lactose…

Q: Transformation: A) O is the process by which a bacterium converts genetic information into a protein…

A: Prokaryotes are the single celled organisms (unicellular) and are the simplest form, which do not…

Q: 1) Which of the following are referred to as retrotransposons? a) VNTRS b) SINES c) LINES d) STRS 2)…

A: As per our honor code, we are authorized to answer only one question at a moment. So, we are…

Q: My Avya mouse is brownish because a) the CpG island with the transposon is unmethylated. b) it was…

A: Epigenetic systems that facilitate gene-environment interactions are involved in the ability of…

Q: a piece of DNA is produced with  1) an enhancer from a gene expressed in the liver, 2) a promoter…

A: Given: A piece of DNA is produced with 1) an enhancer from a gene expressed in the liver, 2) a…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: Where does RNA polymerase bind DNA?a) Promoterb) Operatorc) Enhancerd) None

A: The DNA is the genetic material that is passed from one generation to the next generation. It is…

Q: this is an example of biotechnology: In order to increase the yield of oil from canola, research…

A: Biotechnology is the branch of science that deals with the production of various products by using…

Q: Select the true statements: A.) It is possible, through genetic engineering, to fuse a fluorescent…

A: Explanation:     -A fluorescent fusion protein can indeed be produced by fusing a fluorescent…

Q: Gene expression in bacteria is controlled by the operon model.  a) Why is the operon model…

A: BASIC INFORMATION  OPERON MODEL  This model of gene regulation was put forward by Francis Jacob and…

Q: An alien visits Earth and is found to have the same 'genetic code' as humans. However, the alien has…

A: Mutation occurs when there is a change or damage in the DNA in such a way that it alters the genetic…

Q: a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an…

A: The question says that transcription is not affected but translation is. Therefore, some sequence…

Q: Codon optimization is a widely used process for recombinant expression in prokaryotic systems.…

A: The codon is comprised of three nucleotides or triplets of bases. A nucleotide refers to a molecule…

Q: If tryptophan is absent from the environment of E.coli, the trp operon will be _______ A) mutated…

A: trp operon It is defined as a group of genes which encode the biosynthetic enzymes for the…

Q: If you made a cDNA library from human brain cells and one from human liver cells would the clones in…

A: The term "clone" refers to an organism or a group of genetically identical organisms that are…

Q: Kan selectable marker plant selectable marker constitutive promoter T-region conjugative transfer…

A: Agrobacterium tumefaciens is a soil bacterium that naturally infects plants and produces tumors by…

Q: In a study, bacterial culture of E. coli was infected with bacteriophage. How- ever, these cultures…

A: A restriction endonuclease is a bacterial enzyme that cuts double stranded DNA by cleaving the…

Q: The lactose operon is likely to be transcribed when _____. A) there is more glucose in the cell than…

A: The initiation of transcription determines the regulation of prokaryotic genes. The regulation of…

Q: When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible…

A: The blue–white screen is a techinque that allows for the rapid and convenient detection of…

Q: CRISPR-Cas9 is used by bacteria as a type of immune system against invading viruses. In recent…

A: The question is asking about the type of DNA repair system that is most likely responsible for the…

Q: How many more genes in the plasmid (besides the insulin cDNA insert) are need to determine which…

A: Insulin is a hormone that helps in reducing the level of blood sugar. In diabetic patients, the…

Q: pick a specific disease that is currently a candidate for gene therapy—(a)briefly describing the…

A: Gene therapy aims at treating the disease at the molecular level, by modifying the genetic material…

Q: You want to produce mass amounts of oxytocin, a small peptide hormone produced by the pituitary…

A: The molecular cloning is a technique of production of transgenic organisms. Here one gene is…

Q: The genes for both the α- and βglobin chains of hemoglobin contain introns (i.e., they are split…

A: Introns are the non active genes present in the DNA.

Q: An operon that encodes enzymes that degrade ampicilin is most likely __________ . A) inducible B)…

A: Option A and B are the answer. An operon that encodes enzymes that degrade ampicilin is most likely…

Q: Most bacterial repressor proteins are allosteric. Which of the following binds with the repressor to…

A: Genes are naturally accessible for transcription in bacteria, but repressor enzymes proactively turn…

Q: Which of the following bacterial operon is not controlled by attenuation? a) Histidine b) Leucine c)…

A: The bacterial operon that is not controlled by attenuation is the arabinose operon. Attenuation is a…

Q: The first publications describing the successful production and intracellular replication of…

A: The production of first recombinant DNA takes place in 1970 with the help of the restriction…

Q: Because genetic engineering involves the modification of an organism's set of instructions, it has…

A: * Genetic engineering means it is the process of using rDNA technology to alter the genes of…

Q: The regulation of the trp operon genes by the Trp repressor protein is an example of ______ gene…

A: The regulation of the trp operon genes by the Trp repressor protein is an example of  negative gene…

Q: a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an…

A: Introduction :- Operon model was proposed by Jacob and Monod in 1961. In this model , they explained…

Q: In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells.…

A: Vector is a DNA sequence which serves as a vehicle for transferring the gene of interest from one…

Q: The lac operon has which of the following characteristics? O 1) usually requires an activator…

A: In Eukaryotes all genes are separate and produce individual mRNAs on transcription. However, in…

Q: Which of the following molecules is a protein produced by a regulatory gene? a) operon b) inducer c)…

A: The correct answer is option (d) repressor. Repressor is a protein produced by a regulatory gene. A…

Q: Which of the following is not an example of a transversion mutation? a) T·A -> C·G b) G·C ->…

A: The objective of the question is to identify which of the given options is not an example of a…

Q: Mutations in the CFTR gene result in cystic fibrosis in humans, a conditions in which abnormal…

A: Cystic fibrosis is a hereditary disorder that basically affects the digestive system and lungs of…

Q: A partial diploid in E. coli is created so that LacI is no longer expressed from the genome and is…

A: Partial diploid E.coli has both chromosomal DNA (i.e. genome) and plasmid DNA, but all the genes are…

Q: In some areas of the world, vitamin A deficiencies lead to a high number of deaths every year. To…

A: Golden rice is a variety of rice produced through genetic engineering to biosynthesize beta-…

Q: 1) Assume you have an operon that is repressible. In this case, the rate of synthesis of repressor…

A: An operon is defined as a DNA functioning unit containing a cluster or group of genes under the…

Q: Under which of the following conditions will transcription of the lac operon occur?a) Lactose…

A: Introduction  All the gene expressions are under high regulation and this is necessary to avoid…

Q: In the trp operon, if a mutation occurred in the gene for the repressor so that it could bind to the…

A: Bacteria like Escherichia coli (a friendly gut bacterium) require amino acids to survive since they,…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.