Bacteria are often the preferred hosts for genetic engineering projects by splicing in novel genes from eukaryotes into plasmids, which are moved into competent bacteria. For instance, the gene for human insulin was isolated and moved into a bacterium, which can now produce the much-needed chemical. Previously, type 1 diabetics had to rely on professionals that gathered insulin from human cadavers, cows, and pigs. In order for this feat of genetic engineering to occur, researchers had to start with an unspliced mRNA transcript for h insulin. Agree/Disagree? Explain your response.

. You are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You have identified the gene and place a copy of the gene on a plasmid in E. coli next to a bacterial promoter sequence. You determine that lots of mRNA is made from your gene in your E. coli system, but the protein produced is larger and doesn't have the same properties as the eukaryotic protein you expected. What mistake have you made and how can you fix it?

Bt eggplant is a genetically-modified eggplant developed by Filipino scientists. It was developed to become pest-resistance containing the gene of the bacterium Bacillus thuringiensis which contains toxins that are lethal to some insects including the pest bollworm. Currently, it is under controversy because of a legal battle between its supporters and those who are against it.   Should the government ban the production, commercialization and consumption of the said Product?

A partial diploid in E. coli is created so that LacI is no longer expressed from the genome and is instead expressed from a plasmid. When this E. coli strain is grown in the presence of lactose and glucose, we should expect that the lac operon will be:   Question 14 options:   Constitutively expressed due to LacI being expressed from a plasmid   Induced due to functional Catabolite Activator Protein (CAP)   Repressed due to functional LacI   Induced due to non-functional LacI   Expressed at low levels due to non-functional Catabolite Activator Protein (CAP)

Which among A - D is false regarding antisense RNAS? A) O they occur in protein coding genes B) O they only affect expression of the protein coding genes in which it resides C) O they bind to mRNA D) O they act as translational control mechanism E) Onone are false, A -D are all true

1) Assume you have an operon that is repressible. In this case, the rate of synthesis of repressor protein must depend on the level of a) co-repressor b) inducer c) both d) neither

In the trp operon, if a mutation occurred in the gene for the repressor so that it could bind to the operator even in the absence of tryptophan____________. a) the inducer cannot bind trpO, so operon gene transcription occurs b) the active repressor cannot bind trpO, so operon gene transcription is attenuated c)the active repressor binds tpO, so operon gene transcription is always repressed d) the repressor binds the corepressor, and operon gene transcription occurs.

Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. Not all steps will be placed. Isolate the human gene that produces factor VIII. Isolate the mRNA of the factor VIII gene. Generate cDNA of the factor VIII gene using reverse transcriptase. Incorrect Insert the factor VIII cDNA into a bacterial vector near a promoter site. Transform the vector into an E. coli bacterium. Human DNA is introduced into the bacterial cell using direct injection by a small needle. E. coli expresses factor VIII. Answer Bank The factor VIII protein is isolated from human tissues.

Describe what would happen to the lac operon in a low-lactose environment and in a high lactose environment. a) How will each of these environments affect gene expression? b) What would happen to the repressor in each case? c) How will each of these environments affect RNA polymerase?

A particular species of butterfly is capable of surviving and reproducing both in open grasslands and in forested habitats. However, in the forested habitats this butterfly  is heavily predated (eaten) by a forest bird species, so the beetle species avoids these areas and is generally found only in grasslands. Which of the following statements are true? Answer choices: A. The butterfly's realized niche includes both grasslands and forested habitats. B. The butterfly's fundamental niche includes only grasslands. C. The butterfly's realized niche includes only grasslands. D. The butterfly's fundamental niche includes both grasslands and forested habitats E. The butterfly and the forest bird species are in competition.