B) How many milligrams are there in a 1cc dose of 2% Xylocaine? C)You have 20% acetylcysteine; how many milliliters do you need of this to form 4 ml of 8% solution?

From the Hill Plot below, the of the first binding event for the receptor-ligand system under study is: Q1 4 nM 10 μΜ -2 nM 2 nM Calculate the Hill Coefficient from the receptor-ligand binding data below: Q2 4 100 2 3 (077) log 10 8 6 4 2 0 -2 -4 -6 -6 -4 -2 0 2 4 log [L] (nM) 6 8 10

Di-isopropyl fluorophosphate is a(n) .................. inhibitor of chymotrypsin ? a) allosteric b) irreversible c) uncompetitive  d) competitive e) mixed

Vitamin K questionsa) What transformation of glutamate side chains is Vitamin K (as its reduced form, VitaminKH2) involved in, and what is the relevance of this reaction to blood clotting?b) How does the blood thinner Coumadin (warfarin) prevent clotting?c) How can the dietary intake of large amounts of green vegetables, such as broccoli or kaleinterfere with the action of Coumadin?

For each of the following examples, indicate whether the drug is acting on physical process, chemical process or enzymatic system (your answer should be only; Physical, Chemical or Enzymatic).   A. A drug is used as an antidote in lead poisoning and acts by binding to lead particles in body (chelation therapy). B. A drug acts to reduce flatulence and acts by reducing the surface tension of intestinal gas bubbles in the GI tract (e.g. Simethicone). C. A drug competes with alpha-glucosidase in intestine to reduce glucose conversion from disaccharides (e.g. Acarbose).

3) The following is the amino acid sequence of bradykinin, a peptide released by certain organisms in response to wasp stings: Arg – Pro – Pro - Gly – Phe – Ser – Pro – Phe – Arg What amino acids or peptides are produced when bradykinin is treated with each of the following reagents? a) with Carboxypeptidase c) with Trypsin b) with Chymotrypsin d) with DNFB

What is the mechanism(s) of action of the drug Riluzole in MND?

H CH₂ H₂C HC-CH3 CH₂ H H₂C (S) H₂C H CH₂ CH₂ CH₂ NH O C NH NH₂ a) Which of the following statements about this peptide are correct? Group of answer choices Treatment of this peptide with trypsin generates two products. This peptide is a substrate for carboxypeptidase A Treatment of this peptide with cyanogen bromide generates a pentapeptide and a tripeptide. Treatment of this peptide with chymotrypsin generates three products. Treatment of this peptide with elastase generates 2 products. None of the above statements are correct. b) What is the sequence of this peptide using one letter abbreviations? c) What is the pH which would correspond to the ionization of the peptide as drawn above? 1, 5, 7, 10, 14

00 A) B) C) D) E) Customized Subject Test 63. Which of the following labeled cells in the photomicrograph shown synthesizes antimüllerian hormone? <-B

A. Which one of the following is an example of affinity chromatography? Select all that apply. a) Hormone – receptor interaction b) Enzyme – substrate interaction c) Antigen-antibody interaction d) All of the above e) Cytochrome C – DEAE matrix B. If your sepharose beads are covalently attached to adrenaline hormone, which of the following protein is most likely purified by affinity chromatography? a) Receptor tyrosine kinase (RTK) b) Calmodulin c) GPCR d) Protein kinase A C. Which of the following malate dehydrogenase (MDH) has a 'peptide epitope tag'? a) MDH-HA b) MDH-GST c) MDH-GFP e) All of them D. To elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate? a) Low salt concentrations b) High salt concentrations c) Adding a soluble ligand that competes with the affinity tagged protein for binding to the column d) Just keep washing buffer through the column, isocratic elution