At 590 nm, crystal violet (a biological stain) has a molar absorptivity (ε) of 8.7×104 M-1cm-1. Using Beer's Law, calculate the concentration of crystal violet in a solution with a measured absorbance (A) of 1.254. The path length (b) is 1.00 cm.

At 590 nm, crystal violet (a biological stain) has a molar absorptivity (ε) of 8.7×104 M-1cm-1. Using Beer's Law, calculate the concentration of crystal violet in a solution with a measured absorbance (A) of 1.254. The path length (b) is 1.00 cm.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry

Section: Chapter Questions

Problem 9.1QAP

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

Question

At 590 nm, crystal violet (a biological stain) has a molar absorptivity (ε) of 8.7×10⁴M^-1cm^-1. Using Beer's Law, calculate the concentration of crystal violet in a solution with a measured absorbance (A) of 1.254. The path length (b) is 1.00 cm.

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Spectrophotometry is a method to measure how much a substance absorbs light by measuring the intensity of light passing through solution.

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