Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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A standard 1 mL cuvette is three-quarters filled with a 1 in 6 dilution of 24 μmol.L-1 oxyhaemoglobin. The absorbance is measured at 600 nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.60, a decrease of 0.35 on the first measurement.

What is the molar absorbance coefficient of the deoxyhaemoglobin at 600 nm? (do not enter the units in the answer, 2 decimal places)

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