Which of the following microscopytechniques can be used in live cell imagingin a live animal.1. Structured illumination microscopy2. Stimulated emission depletionmicroscopy3. Photoactivated localization microscopy4. Two-photon excitation microscopy5. Total internal reflection microscopy6. Light-sheet microscopyO4 and 6O2, 3 and 6O1 and 43 and 5O2 and 4

Live cell imaging within live animals is a vital facet of contemporary biological research, enabling…

Answered: Which of the following microscopy…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

1. How will the following affect resolution during microscopy?i. Closing or opening the diaphragmii. Raising or lowering the condenserii. Increasing or reducing the light intensity 2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate: i. The calibrated value of the ocular micrometer in um.ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions. 3. What are the methods of staining?
1.Why is wavelenth the main limiting factor on limit of resoltuin in light microscopy? 2.Assuming that all other variables remain constant, explain why light of shorter wavelengths will produce a clearer image that light of longer wavelenths. 3. Why aren't the magnification of both ocular lenses of a binocular microscope used to calculate total magnification?
1. Differentiate the compound light microscope from the dissecting microscope in terms of: *image produced *magnification *type of specimen examined 2. Write down the principles of the following: *electron microscopy *scanning tunneling microscopy *atomic force microscopy
2. Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A.) Features (color of the band, diameter of the opening of the lens, etc.) LPO HPO ΟΙΟ
1. Identify features of Dark-field Microscopy (1-2 paragraph) 2. Give three examples of the uses of microscopes outside the field of biology and medicine. (3 examples with 5sentences explanation each)
1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?
1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
Describe 3 simple ways of preparing slides for light microscopy microscope
II.VIRTUAL MICOSCOPE 4X 4X SPECIMEN 10X 10X 40X 40X 100X 100x COARS FOCUA TME FOOR LCHT AVAN 100X MAGNIFICATIO | 4X N USED IN THE VIRTUAL MICROSCOPE OCULAR MAGNIFICATIO (10X) TOTAL MAGNIFICATIO
3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.
4) Answer true or false to each of the following statements. On high power, you should use the coarse adjustment knob. The diaphragm determines how much light shines on the specimen. The low power objective has a greater magnification than the scanning objective. The fine focus knob visibly moves the stage up and down. Images viewed in the microscope will appear upside down. If a slide is thick, only parts of the specimen may come into focus. The type of microscope you are using is a scanning microscope. For viewing, microscope slides should be placed on the objective. In order to switch from low to high power, you must rotate the revolving nosepiece. The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.

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