Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope.At 100X you are unable to see the diatom. Explain why.Explain what correction (s) should be made to allow you to see the diatom using the 100X lens.After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred. Figure 1: Diatom observed using the 10X objective lens.

Microscopy is the evaluation of the sample/tissue/cell with the help of microscope. It generally…

Answered: Assume you are observing the diatom…

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter24: Informatics In Medical Imaging

Section: Chapter Questions

Problem 8RQ

Related questions

Question

Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope.
1. At 100X you are unable to see the diatom. Explain why.
2. Explain what correction (s) should be made to allow you to see the diatom using the 100X lens.
3. After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred.