As we focused on the translation of mRNA into proteins as well as on protein structure and function. Along the way, we found many opportunities to consider the methods and reasoning by which much of this informationwas acquired. From the explanations given in the chapter,what answers would you propose to the following fundamentalquestion What experimentally derived information led to Holley’sproposal of the two-dimensional cloverleaf model of tRNA?

What is an anticodon and where is it located on the tRNA structure?

In the absence of cladosporin, explain the elongation steps in the synthesis of lysyl-tRNA synthetase enzyme or protein in bacterial cells by including any elongation factors, base pairing of codon/anticodon, any conformational shift, proofreading, any hydrolysis, exchange, ribosomal subunits involved, charged tRNA, peptide bond formed.  This question does not require a super long in depth answer, a short to the point answer is preferred if possible. Thank you!

Name the four classes of RNA and briefly explain their functional significance. In what reaction(s) do they participate?

In regard to the wobble hypothesis and the fact that cells do not need a full complement of tRNAs for codons. What would the consequences be if a cell lost the tRNA gene with the following anticodon sequences Please explain in depth A. 3'-ACA-5' B. 3'-CUG-5'

What are two possible anticodon sequences for tRNA's that are charged with Phenylalanine (Phe)? O 3' AAU 5' and 3' GAA 5' O 3' AAA 5' and 3' AAG 5 3' UUG 5' and 3' CUG 5' O 3' GAA 5' and 3' CAG 5' 3' GUA 5' and 3' GUG 5'

Examine the tRNA in Figure 15.14. What do you think would be the potential effect of a mutation in the part of the tRNA gene that encodes (a) the acceptor stem; (b) the anticodon; (c) one of the red nucleotide positions?

IX. Dibuje la estructura del polinucleótido que aparece a continuación el cual corresponde a un codón en el mRNA que codifica para el aminoácido prolina. (Solamente se pide el dibujo de la estructura siguiendo el modelo de polinucleótido mostrado en clase). e). .) 5'-C-C-C-3' Show Transcribed Text IX. Draw the structure of the polynucleotide below which corresponds to a codon in the mRNA that codes for the amino acid proline. (Only a drawing of the structure following the polynucleotide model is requested)

Explain how lysyl-tRNA synthetase protein in bacterial cells typically undergoes folding with necessary structures and steps, any ATP hydrolysis, cap structure, etc.

Treating a solution of ribonuclease with 2-mercaptoethanol and urea denatures the enzyme. If the 2-mercaptoethanol is removed, followed by the removal of urea, the ability of the enzyme solution to hydrolyse RNA is reduced by about 99%. What is the most plausible explanation for this observation?   a. All of the RNase molecules have about 1% of their usual activity, due to changes to the organisation of disulphide bonds   b. Random formation of disulphide bonds mean about 1% of the RNase molecules are 100% active whereas 99% have no activity   c. Deprotonation of histidine in the active site of the enzyme means that only about 1% of the enzyme molecules are active   d. Deprotonation of histidine in the active site of the enzyme means that all of the enzyme molecules have 1% of their usual activity