As seen in the illustration below, you inserted your desired gene into pBR322. You used BamHI to splice the vector and insert. After ligation and transformation, explain your screening methods. Scal 3844 Pvul 3723 Pstl 3607 Vspl 3537 Eco311 3433 Eam11051 2361 Cail 2884 rep (PMB1) bla (Ap) 3293 3133 Sspl 4168 Bsul5) 23 EcoRI, Xapl 4259, Hindi 29 Eco321 185 Aatil 4284, All, Pacl 2473 2519 Lgul 2250 4153 pBR322 4361 bp Ndel 2296 Bst11071 2244 1915 Bspol, Nhel 229 BamHI 375 гор Pull 2064 Piol 2117 Esp31 2122 Ppu211 2225 Psyl 2217 Tsti 389 SgrAl 409 tet (TC) 1276 Pael 562 Xagl 622 Sall 651 Boxl 712 Eco521 939 Bsp681 972 Bvel 1063 Mva12691 1353 Eco1301 1309 Eco881 1425 Misl 1444 Bpu101 1580 Bagl 1650 Kpn21 1664 BseJl 1668

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Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.