Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? inning?)
Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? inning?)
Chapter25: Diuretics And Medications Used For Urinary System Disorders
Section: Chapter Questions
Problem 22RQ
Related questions
Question
Transcribed Image Text:Chart 3: Pepsin digestion of Protein
Tube No.
Additives
1.
Pepsin
2
3.
Pepsin
BAPNA
4.
water
5.
Pepsin
BAPNA
9.
Pepsin
Pepsin
BAPNA
water
BAPNA
pH 2.0
Buffer
BAPNA
pH 2.0
Buffer
Boil first,
Incubate
37°C
pH 2.0
Buffer
37°C
pH 2.0
Buffer
pH 7.0
Buffer
pH 9.0
buffer
Incubation
37°C
37°C
37°C
37°C
Condition
(all 60 min.)
Predict
results
(before lab)
Optical
density
Answer Questions about your results:
1. Which pH provided the highest pepsin activity?
Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands?
2. Would pepsin be active in the mouth?
Explain your response:
3. What are the products of protein digestion (subunits of proteins)?
4. Why is tube 4 described as "control" for this experiment on effect of pepsin? (hint -what is missing?)
5. Tubes 1 and 2 contained the same substances. Explain why their optical density measurements
were different:
6. If you had not run the tube 2 and tube 3 samples, what argument could be made against the
statement "Pepsin digests BAPNA"?
7. How did your predictions compare with actual results?
Explain:
Additional Activity:
Find your BMI:
- using your height & weight- chart can be found at Mayo Clinic site:
https://diet.mayoclinic.org/diet/eat/what-is-your-bmi?xid=nl_MayoClinicDiet_20160426
**At home or at end of lab: Do review Q's PhysioEx#8 (Activity 1: 1-5, Activity 3: 1-3,)**
9.
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