The regulation of Glutamine Synthetase (GS) in E.coli, a model for this highly conserved homodecamer in mammals, and is a striking example of cumulative feedback inhibition through allosteric regulation. Explain the cumulative feedback inhibition of GS..

Assume that the gene for pyruvate dehydrogenase kinase is mutated and the mutatedenzyme remain active in the presence or absence of upstream regulatory signals. Patients withsuch a gene mutation exhibit all the symptoms of beriberi. Do you think beriberi, resulting fromsuch mutations of pyruvate dehydrogenase kinase, can be successfully treated by placing suchpatients on a thiamine-enriched diet? Briefly explain your reasonings.

Why does enzyme x catalyze the formation of the phosphodiester bond and please explain how nucleas recognizes phospjodiester bonds

What is the role of the aspartate 118 residue in the Human inorganic Pyrophosphatase mechanism? include a drawing of the mechanistic proposal.