Answer Bank Glyceraldehyde 3-phosphate + P¡ + NAD+ →→→ 1,3-bis 3-Phosphoglycerate →→→ 2-phosphoglycerate 2-Phosphoglycerate phosphoenolpyruvate Pyruvate + NADH + H+ →→→→→lactate + NAD+ — 1,3-Bisphosphoglycerate + ADP →→→ 3-phosphoglycer Phosphoenolpyruvate + ADP →→→ pyruvate + ATP

The mechanism of chymotrypsin can be viewed as a two-step process, acylation of the enzyme active site followed by a deacylation reaction. What accounts for the observed "burst" in rapid kinetic studies of the hydrolysis of N-acetyl-L-phenylalanine p-nitrophenyl ester by chymotrypsin? The rate of hydrolysis of the acyl-enzyme intermediate is faster than the rate of forming the acyl-enzyme intermediate. The rates of the acylation and deacylation reactions are equal. The rate of the acylation reaction is slower than the rate of the deacylation reaction. The rate of the acylation reaction is faster than the rate of the deacylation reaction.

For the following enzymes (3-6) predict how the conditions will most likely affect the enzymes activity with one of the following and provide a 1 sentence explanation: a. increase activity                     b. decrease activity                             c. not likely to alter activity  3) alpha-ketoglutarate dehydrogenase complex binding to AMP when [AMP] is high. 4) phosphohexose isomerase binding NADH when [NADH] is high 5) phosphofructose-1 binding NAD+ when [NAD+] is high  6) pyruvate dehydrogenase complex binding to ATP when [ATP] is high

In different organisms sucrose can be cleaved either by hydrolysis or by phosphorolysis. Calculate the ATP yield per mole of sucrose metabolized by anaerobic glycolysis starting with (a) hydrolytic cleavage and (b) phosphorolytic cleavage.