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Absorbance 2.5 Standard Curve y= 2.6632x -0.0333 2 15 1 0.5 0 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 Concentration (ug/mL)

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter1: Basic Mathematics

Section: Chapter Questions

Problem 8RQ

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Question

You had a second solution with an unknown concentration of Protein X that had to be diluted 4x before you could measure the absorbance. The diluted solution had an absorbance of 0.76. What is the concentration of the diluted solution in ug/ml and what is the concentration of the original solution

Absorbance
2.5
Standard Curve
y= 2.6632x -0.0333
2
15
1
0.5
0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
Concentration (ug/mL)

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