A standard containing 1.0 ppm NO3-N was treated with a reactive agent to give the absorbance of the 0.30 at 600 nm. A sample was analysed in same manner, which gave absorbance of o.15 at the same wavelength. Quantify the level of NO3-N in this sample: 0.45 ppm NO3 O 0.75 ppm NO3 0.50 ppm NO3 non of above

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.