A series of standards of a rhodamine derivative were measured with an excitation wavelength of 480 nm (2ex) and an emission wavelength of 620 nm (em). The following data was obtained: (i) (ii) (iii) Standard 1 2 3 4 5 Rhodamine Derivative Concentration (ppm) 1.9 4.0 5.8 8.2 10.0 Emission Intensity 1100 2150 3130 4350 5900 Explain the difference between the excitation wavelength and the emission wavelength. Using the data provided, determine the extinction coefficient in dm³.mg¹.cm¹ and the molar extinction coefficient in dm³.mole¹.cm¹. The rhodamine derivative has a molar mass of 480 g.mole-¹. If the unknown sample has a fluorescence emission intensity of 3899, calculate the concentration of the sample in parts per million (ppm) and moles dm-³ (molarity).

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A series of standards of a rhodamine derivative were measured with an excitation
wavelength of 480 nm (ex) and an emission wavelength of 620 nm (em). The
following data was obtained:
(i)
(ii)
(iii)
Standard
1
2
3
4
5
Rhodamine Derivative
Concentration (ppm)
1.9
4.0
5.8
8.2
10.0
Emission
Intensity
1100
2150
3130
4350
5900
Explain the difference between the excitation wavelength and the emission
wavelength.
Using the data provided, determine the extinction coefficient in dm³.mg¹.cm-¹
and the molar extinction coefficient in dm³.mole¹.cm¹. The rhodamine
derivative has a molar mass of 480 g.mole¹.
If the unknown sample has a fluorescence emission intensity of 3899,
calculate the concentration of the sample in parts per million (ppm) and
moles dm³ (molarity).
Transcribed Image Text:A series of standards of a rhodamine derivative were measured with an excitation wavelength of 480 nm (ex) and an emission wavelength of 620 nm (em). The following data was obtained: (i) (ii) (iii) Standard 1 2 3 4 5 Rhodamine Derivative Concentration (ppm) 1.9 4.0 5.8 8.2 10.0 Emission Intensity 1100 2150 3130 4350 5900 Explain the difference between the excitation wavelength and the emission wavelength. Using the data provided, determine the extinction coefficient in dm³.mg¹.cm-¹ and the molar extinction coefficient in dm³.mole¹.cm¹. The rhodamine derivative has a molar mass of 480 g.mole¹. If the unknown sample has a fluorescence emission intensity of 3899, calculate the concentration of the sample in parts per million (ppm) and moles dm³ (molarity).
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