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- H2N 1.) Look carefully at this nucleotide: N: N- Но-Р-О OH a.) Number the carbons in the sugar group. (Remember the "prime" symbols.) b.) Is this a purine or a pyrimidine? How do you know? c.) Would this nucleotide be used for DNA or RNA? How do you know? (Be specific.) d.) Is this nucleotide ready to be used for DNA replication or RNA transcription? Why/why not? e.) If this nucleotide were incorporated into a growing DNA or RNA strand, where would the next added nucleotide be attached to this one?VII. Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the following significant information: Complete hydrolysis of the peptide yielded equimolar amounts of Leu, Orn, Phe, Pro, and Val Molecular weight of the peptide was estimated to be aroung 1,200 Da The peptide failed to undergo hydrolysis when treated with carboxypeptidase Partial hydrolysis and then chromatographic separation of products yielded the following di- and tripeptides: Leu-Phe, Phe-Pro, Orn-Leu, Val-Orn, Val-Orn-Leu, Phe-Pro-Val, Pro-Val-Orn Treament with dinitrofluorobenzene (DNFB) followed by complete hydrolysis yielded only free amino acids and the DNFB-derivatized ornithine at its side chain Using this set of information available to you, deduce the amino acid sequence of this peptide antibiotic. Write your final peptide sequence only using 3-letter abbreviations. /b. What is the difference between the 3' and the 5' ends of a nucleotide chain? C. Do the chains run the same way? d. How are the chains connected? e. Which bases bond to each other? f. What kinds of bonds hold the chain together? 3. What are the main differences between RNA and DNA? 4. Distinguish between the structure of pyrimidines and purines. Explain why adenine bonds only to thymine. 5. Name the five nitrogenous bases in the table below, and put an X in the correct column for each base. Then indicate if the base if found in DNA (D), RNA (R), or both (B) hp
- Choose from the options A.The structure of Zovirax is an analog of Thymine Guanine Adenine Uracil Cytidine B.What is the difference of the structure of Zovirax® to the conventional Nucleic acid base It contains ribose It contains an open sugar moiety It contains deoxyribose There is a substituted amino group The amino group is not chiral C.The structure of Zovirax® competitively inhibits RNA Poymerase DNA Polymerase Reverse Transcriptase Alkaline Phosphatase Amino acyl transferase D.Zovirax® is effective against the strain of Varicella Herpes simplex virus Vesicular Stomatitis Virus All None E.This agent is the least active among all fluoroquinolones Levofloxacin Ciprofloxacin Moxifloxacin NorfloxacinN. NH 2. One of the key pieces of information that Watson and Crick used in determining the secondary structure of DNA came from experiments done by E. Chargaff, in which he studied the nucleotide composition of DNA from many different species. O=P-OCH, N. `NH, HN он O= P- OCH, NH, Chargaff noted that the molar quantity of A_was always approximately equal to the molar quantity of T. and the molar quantity of C was always approximately equal to the molar quantity of G. How were Chargaff's results explained by the structural model of DNA proposed by Watson and Crick? N OH N. O= P-OCH, OH OHIndicate which of the following descriptions does not apply to the biochemical depicted here: O 10-4-0- O™ O O || HO-P-O-P-O-P-O O™ a. DNA building block b. Complementary to dGTP c. Complementary to dATP d. Base is a pyrimidine OH NH
- Please give a clear handwritten answer only with explanation..Draw 5’-A-A-3’ ribonucleotide with 5’ phosphate and 3’ hydroxyl4. A. Draw the structure of the following peptide sequence at pH 2.0: ASP-ALA-THR-LYS-GLY B. What is the net charge of this pentapeptide at pH 2.0?u ivercarbUIT sugar, a phosphate group, and à nitrogenous base. 35.Most antimicrobial drugs currently in clinical use offer a great variety of unique targets for selective toxicity. Macrolides are broad-spectrum, bacteriostatic drugs that block elongation of proteins by inhibiting peptide bond formation between specific combinations of amino acids. Azithromycin is a broader spectrum antibiotic that is able disrupt INITIAL peptide bond formation. Which of the following protein structures will be altered, FIRST, by Azithromycin? a. the quaternary structure will be disrupted b. the secondary structure will be disrupted c. the tertiary structure will be disrupted d. the primary structure will be disrupted e. no levels of protein structure will be disrupted
- 1. Choose the proper structure of the predominant form of L-M-L-P-D-T at pH 5. Provide a short explanation. 2. Choose the proper structure of the predominant form of L-A-L-Y at pH 11. Provide a short explanation.a. Why is it important to eat food containing antioxidants? Write at leasttwo reactions to prove the answer. b. Write in four ways the following nucleotide sequence: ATGCA. c. Explain i. Hoogsteen pairing & ii. Hyperchromic effectiii. Epimers Iv. Mutarotaton v. Aldose vi. Anomers vii. Mutarotation1)What is the objective of hydrolyzing the RNA prior to doing biochemical tests? 2) What particular test, other than Molisch's, may be performed to determine the presence of pentose sugar? Create a process. 3) List the sugars, purines, and pyrimidine bases found in DNA and RNA and write down their structural formulae.