A novice microbiology student incorrectly explains that immersion oil increases the magnification of his microscope. What is the function of immersion oil?
Q: Explain how to correctly use the microscope using oil immersion.
A: Light tends to bend while entering one material from a different one. The refraction of light…
Q: Which type of microscope(s) would be used to examine the following specimens? Explain each of your…
A: 1. Atomic force microscope : The atomic force microscope has formed into a useful asset in primary…
Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…
A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…
Q: What kind of microscope was invented in 1932 that was able to record images of transparent…
A: A microscope is a laboratory instrument used to examine objects that are too small to be seen by the…
Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…
A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…
Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…
A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…
Q: The laboratory that you are working in does not have a microscope or motility medium. Now, how will…
A: The motility of a bacterium is determined by the presence and absence of flagella. The flagellum is…
Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?
A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…
Q: Explain the differences that you have seen using types of microscopes such as scanning electron, low…
A: It is an optical device that consists of high definition lenses with the help of which very small…
Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…
A: Option b
Q: You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you…
A: In microscopy, oil immersion is a technique used to increase the resolving power of a microscope.…
Q: Examples of bacteria observe in the microscope using Gram stain, Ziehl-Neelsen, and Kinyoun Method.
A: Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen…
Q: This type of microscope is use to study the detail in samples. It produces a three-dimensional image…
A: A microscope is an optical instrument, which is used for observing small organisms and cells. It…
Q: What features of your Gram stain exemplify the Gram stain theory?
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: When performing a gram stain, what does it tell us about the bacteria and the cell wall speficially?
A: Gram staining is a common approach for distinguishing two broad groups of bacteria based on the…
Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…
A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…
Q: Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of…
A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: The resolution of a microscope could be increased by raising the condenser to its highest point.…
A: Resolution of a microscope is defined as the ability of a microscope to separate two closely spaced…
Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the…
A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal…
Q: If you prepared a sample of a specimen for light microscopy, stained it with the Gram stain, and…
A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…
Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: microscope
A: Microscope helps to see the small things clearly
Q: Which stains are selective in microbiology?
A: INTRODUCTION Gram staining, acid fast staining, capsule staining and volutine granule staining are…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: The specimen for an electron microscope is always a. stained with dyes c. killed b. sliced into thin…
A: Electron microscope: It is a microscope which obtains a high-resolution image of non-biological as…
Q: what is Microscopy and Staining of Microbes
A: Microbes are the tiny unicellular ,prokaryotics organisms having diverse varieties and habitats.…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and…
A: Acid Fast staining is used to detect the presence of acid-fast bacteria e.g. Mycobacterium…
Q: Microbiology When using the Microscope in a lab, the use of oil prevents_____________ (use the…
A: Resolution refers to the ability to distinguish two adjacent objects with accuracy. If the…
Q: Give the functions and magnifications of transmission electron microscope and scanning transmission…
A: Transmission electron microscope and scanning electron microscope are two types of electron…
Q: Why do we use oil with microscope ?
A: ••In light microscopy, in order to increase the resolving power we use oil Immersion techniques
Q: You use gram staining procedure to stain a bacterium that lacks a cell wall. What color will the…
A: Gram staining is a special technique in which bacteria are stained with gram stain. It is used to…
Q: Which Two types of electron microscopy are in routine use in microbiology? Explain
A: The electron microscope is an instrument that uses the beam of electrons to examine objects on a…
Q: Evaluate the following preparations in terms of providing informationon microbial size, shape,…
A: Staining is an important step in observing the microbes under a microscope. they allow to the…
Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…
A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…
Q: discuss the microscopy technique that gives the best contrast of nonbiological samples
A: Microscope is used those objects which are not visible under naked eye it is able to view the finer…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: agnification is required to observe microorganisms?
A: A microorganism, often known as a microbe, is a microscopic organism that can be a single cell or a…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: Endospores can be seen as refractile structures in unstained cells and as colorless areas in…
A: Endospores are dormant, non-reproductive structures formed by certain bacteria during unfavorable…
Q: Why should freshly collected samples be used for microscopic examination?
A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…
Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…
A: The gram-positive and gram-negative bacteria can be differentiated based on their cell wall elements…
Q: what microbial features would you use a scanning electron microscope and transmission electron…
A: Scanning electron microscope special features- projects 3D image of surface Appearance of the…
Q: Describe the use of microscopy to identify microorganisms in patient specimens
A: A microorganism, often known as a microbe, is a minute living entity. Microbiology is the study of…
Q: Why do we stain some organisms before viewing them under a microscope? (see top of p 14)
A: Microbiology is the study of microorganism. The organisms that cannot be seen through naked eyes are…
Q: 5 1 2 6. 4 7 8.
A: Introduction A microscope is a scientific tool that is used to study items that are too small for…
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- For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are the sources of error in performing Gram staining?What are the disadvantages of doing a total microbial count by examining wet mounts under the microscope?Please discuss why staining is important in visualizing microbes under the microscope. What different types of staining methods can be used?
- Why is it sometimes difficult to attain both good resolution and good contrast at the same time for a sample that is being observed on the microscope stage? Please give a thorough response,Explain how to correctly use the microscope using oil immersion.Why would specimens viewed with a compound microscope be thin and/or chemically cleared?
- (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside from bacteria, can gram staining be used with microbes such as fungi? Explain your answer.What is a step-by-step process of preparing and inspecting a microorganism under a microscope?How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity
- How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage controlBefore viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).Identify and describe at least three types of microscopes that can visualize cellular activities and movement or location of molecules, including the stains being used. Describe elaborately other stains being used in microbiology.