(a) In High Performance Liquid Chromatography (HPLC), the separation method is by partition of the solute (analyte) between the mobile phase and the stationary phase. Explain how this leads to separation of analyte mixtures; (b) Describe the structure and materials use in a typical column used for HPLC. Thereby explain why high pressures have to be used in HPLC; (c) Discuss how the need for high pressures also imposes requirements on the injector;
(a) In High Performance Liquid Chromatography (HPLC), the separation method is by partition of the solute (analyte) between the mobile phase and the stationary phase. Explain how this leads to separation of analyte mixtures; (b) Describe the structure and materials use in a typical column used for HPLC. Thereby explain why high pressures have to be used in HPLC; (c) Discuss how the need for high pressures also imposes requirements on the injector;
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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