A fermentation process was carried out with a yeast in a chemically defined medium containing glucose (C6H12O6) as a carbon source aiming at the production of ethanol (C2H6O). For the evaluation of the process, samples were taken at different times of cultivation and the experimental results are shown in the Table below. Based on the experimental results, one can:   a) Calculate the overall substrate-to-cell conversion factor

Biology Question

What medium is this? What is the result in tube at left? In second tube? What does it tell you about the biochemical abilities of these organisms? What is the reagent used to detect the red?

What can be interpreted when the Benedict's test is applied to enzymatic hydrolysis of sucrose (yeast- hydrolyzed sucrose)?

Explain why phenol red is used in McConkey agar to detect the acid reaction of lactose fermentation.

Biochemical tests such as the fermentation test can be used when unknown bacterial cells are identified. true or false?

A microorganism is grown in a fermenter under steady-state conditions. The microorganism growth follows Monod kinetics and the following stoichiometric equation: C3H6O3 +a O2 +b NH3 → c biomass + d CO2 + e H₂O Given that the biomass yield is 0.415 grams of biomass per gram of substrate, determine the respiratory quotient.

A purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The reaction volume was 145.0 µL and the final volume before loading the plate was 1,050 µL. The total reaction time was 4.25 min. The amount of protein used in the reaction was 4.270 µg. Calculate the specific activity of the sample (in nmol min-1 µg-1).

Outline the steps involved in the two paths of sulfate assimilation.  Be sure to address the sulfur reduction pathway as a photoassimilation process.

roblem 1An aerobic biochemical process uses a CSTR w/o recycle. The feed characteristics are asfollows: Influent substrate concentration, So = 200 mg/L; half- velocity coefficient, Ks = 50mg/L; maximum, specific substrate utilization rate, k = 5 g/g- day; yield coefficient, Y = 0.5g Xa/g substrate consumed; microorganism decay coefficient, b = 0.10 day-1.1. The process goal is the production of active biomass. Determine the hydraulicretention time that the reactor should be operated in order to maximize the reactoractive biomass concentration (Xa). How does this retention time compare with theminimum solids retention time that the system can theoretically be operated?Calculate the solids retention time safety factor.2. Based on the solids retention time for maximum Xa calculated above, estimate theactive biomass concentration (Xa) and the substrate removal efficiency (E, %)