(a) Explain why fluorescence spectroscopy gives a more accurate determination of vitamin B1 than absorption spectroscopy. (b) Provide an annotated sketch of the excitation/emission spectrum as well as an annotated energy profile diagram of the vitamin B1 derivative, thiochrome.
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- What are the advantages and disadvantages of HCR-20 for use in clinical services? I need this to be detailed and thoroughly explained with examples and support from research evidence. Thank you :) 4Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Fully excluded Partially included Fully included the activity of lysozyme was determined by looking at the increase in light scattering per unit time increase in absorbance per unit time. decrease in absorbance per unit time. increase in concentration per unit time.Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…
- You are stumped for a while, because you really don't want to spend all day running columns, and then having to measure the column fractions with UV-VIS. Your colleague however tells you that it is possible to use the spectrum of the mixture to determine the concentration of cach vitamin in the mixture. (c) Briefly explain why it is possible to use the spectrum of the mixture to determine the concentration of each vitamin in the mixture. Now that you realise that it is possible, you get some information about the molar absorptivities of cach vitamin at cach Amax, and list it in Table 1. Table 1. The molar absorptivities of Vitamins B1 and B6 measured in pure samples of each. E (M' cm') a (nm) Vitamin B1 Vitamin B6 423 5075 240 11280 285 1504 (d)(1) Use the information in Table 1 and the absorbance of the sample mixture at the relevant wavelengths (Figure 1) to determine the concentration of Vitamin B1 in the diluted, prepared sample. (d)(i) Determine the mass of Vitamin B1 in one of…A70-year-old woman with a long history of systemic lupus erythematosus has been receiving prednisone for the past 3 months. She also has been receiving captopril to treat her hypertension. Which of the following laboratory results would be evident in this patient? ???? ?????? ?? ????? ?? ???? ???????? a. Hyperglycemia b. Hyponatremia c. Hypercalcemia d. Hypocholesterolemia e. Hypokalemiamacrophages Question 48 Please describe/name the structural variants describe in the picture: Courtesy of CRC Press/Taylor & Francis Group А. 1 ACGCTGCGGTTCGATAGT 2 ACGCTGCG-TTCGATAGT В. 1 ACGCTGCGGTT-CGATAGT ACGCTGCGGTTTCGATAGT C. 1 ACGCTGCGGTTCGATAGT ACGCTGC CA GATAGT D. 1 CACACACACACACACACACACACA 2 CACACACACACACACACACACA E. 1 100 100 | 100 100 2 100 100 100
- e Edit View Insert Format Tools Add-ons Help Last edit was 2 minutes ago 47 90% ▼ Normal text ▼ Comic San... ▼ 1 11 + B I UA C 1 1 T 2 3 4 | 5 * 6 9) What Is the effect of exercise on BP? How does the body benefit from this change in BP during exercise? 10) How would the BP of an anxious patient visiting a doctor be different than if the patient is calm? 11) In atherosclerosis, plaque builds up inside the arteries. How would this affect BP? Is this an example of hypertension or hypotension?|1000 Tablets 48, Kandivli Ind Estate, Mumbai 400 067, India by: Ipca Laboratories Limited Jacksonville, FL 32257 USA Ranbaxy Pharmaceuticals Inc. Manufactured for Rx only LOT: EXP: 100 mg TABLETS, USP ALLOPURINOL N Each scored tablet contains: Allopurinol, USP........100 mg Store at 20° to 25°C (68° to 77° F) [See USP Controlled Room Temperature]. Dispense in tight, light-resistant container as defined in the USP. USUAL DOSE: One tablet daily. For indications, dosage, precautions, etc., see accompanying package insert. Code: DNH/DRUGS/NH/170 3 633041153910 NDC 63304-539-10 Non varnish area R RANBAXY LBZTL6 0311 Expiration date Prescription warning Total amount in vial, packet, box Usual adult dose Dosage strength Drug form Lot number (control number) National Drug Code (N D C) # Manufacturer Brand name Generic nameGM is a 55 yo that just returned from visiting their sister in Arizona. They got a sunburn while out playing tennis and are seeking advice on how to manage their sunburn. The pharmacist looked at the patient's sunburn and gathered the following information from them: • NKDA (sensitive to adhesives and some topical anesthetics) • No current medications or medical conditions S - tightness and redness of the burnt skin without blistering; denies fever, chills, headache, or tiredness C - skin was only slightly pink yesterday, has turned deeper pinkish red today H - they haven't done anything to treat it so far O - burn appeared today (the morning after being outside) L - skin on their arms (just above elbow to wrist) and legs (midthigh to top of ankles) and back of neck where their tennis shirt and shorts did not adequately cover them; they were wearing a baseball hat and sunglasses. No burn present on face. A - mild pain especially when the skin is touched or brushes against clothes or…
- after a 5 g onion sample is divided into two equal parts, heat treatment to one of these partsit is being implemented. Then both heat-treated and non-treated parts are 10 times their weightit is ground in an environment containing as much buffer and the determination of pyruvate in the supernatants obtainedit's being done.a) As a result of measurements taken at 520 nm; absorbance value for the heat-treated sample;it was found that the absorbance value in the sample that was not heat treated was 0.123 and the absorbance value in the sample that was not heat treated was 0.520.The equation of the calibration accuracy for pyruvate is that y = 0.1367x – 0.001 (nmol/mL)according to the onion sample; a) independent of alinase activity; b) dependent on alinase activityand c) calculate the total pyruvate amounts in terms of nmol/g of onion.b) How is there a relationship between the activity of the enzyme allinase in onions and the amount of pyruvate?Dec,please explain.A Greek pharmacist namely Claudius Galen is honored from his work called ____ which has similarities in cold cream? Glycerin suppositories can be used for adult or infant or both. explainSuppose a city’s water supply has 0.2 ppb (1ppb=10-3 mg/L) of polychlorinated biphenyls (PCBs) in it. Using the PCB oral slope factor (7.7 (mg/kg-d)-1 and the EPA recommended exposure factors given in Table 6.14 below, (a) What would be the individual lifetime cancer risk for an adult male residential consumer?(b) In a city of 1 million people, use this risk assessment to estimate the number of extra cancers per year caused by the PCBs in the water supply. “Extra” cancers refers to cancers caused by PCBs (as opposed to other causes such as smoking, etc.)(c) Assume the average cancer death rate in the United States in 193 per 100,000 per year. How many cancer deaths would be expected in a city of 1 million? Do you think the incremental cancers caused by PCBs in the drinking water would be detectable?