A diagram of a gene is shown below. Normally, exons 1, 2, and 3 are present in the mature mRNA. (shown in attatched image) A mutation occurred at the 5’ splice site of exon 1 as shown by the star such that the spliceosome cannot bind to this location during mRNA processing of the pre-mRNA into the mature mRNA. Which of the following is/are a possible result of this mutation? (select all that apply) a)The pre-mRNA transcribed from this gene sequence will be longer than normal. b)The protein translated from this mRNA will be a different length than the normal protein. c)The mature mRNA will be longer. d)The mature mRNA will be shorter.

You are studying the effect of various mutations in this gene. The AG sequence (3’splice site) that is within intron 2 [adjacent to Exon 3] has been changed to a CC.  What would the resulting mRNA transcript look like? Draw a similar diagram as above and indicate the nucleotide lengths on the mRNA transcript.

A common feature of many eukaryotic mRNAs is the presence of a rather long 3′ UTR, which often contains consensus sequences. Creatine kinase B (CK-B) is an important enzyme in cellular metabolism. Certain cells—termed U937D cells—have lots of CK-B mRNA, but no CK-B enzyme is present. In these cells, the 5′ end of the CK-B mRNA is bound to ribosomes, but the mRNA is apparently not translated. Something inhibits the translation of the CK-B mRNA in these cells. Researchers introduced numerous short segments of RNA containing only 3′ UTR sequences into U937D cells. As a result, the U937D cells began to synthesize the CK-B enzyme, but the total amount of CK-B mRNA did not increase. The introduction of short segments of other RNA sequences did not stimulate the synthesis of CK-B; only the 3′ UTR sequences turned on the translation of the enzyme. On the basis of these results, propose a mechanism for the inhibition of CK-B translation in the U937D cells. Explain how the introduction of short…

Which of the following mutations in the protein-coding region of a gene is more likely to lead to complete loss of function of the encoded protein: an insertion of six nucleotides or a deletion of two nucleotides? Briefly explain your answer.

A monocistronic mRNA is defined by… Group of answer choices The presence of a 5’ triphosphate The absence of introns The presence of one coding sequence The absence of a poly(A) tail   Eukaryotic mRNAs are protected on the 5’ end by… Group of answer choices A methylated guanosine A polymer of adenine A triphosphate RNA primer   Eukaryotic mRNAs are typically processed in this order: Group of answer choices Introns spliced out, poly(A) tail added, capped Poly(A) tail added, capped, introns spliced out Capped, poly(A) tail added, introns spliced out Capped, introns spliced out, poly(A) tail added

The diagram, shown in attatched image, shows an mRNA that is alternatively spliced. The alternatively spliced variant contains Exon 1, Exon 2, and Exon 4. Indicate below if a splice site is used, blocked by a modifying protein such that the spliceosome must choose a different site, or skipped to make this alternatively spliced variant.

For each of the following, identify whether that sequence or feature of a typical protein-coding gene would be recognizable in the specified molecule in a typical prokaryotic cell. 5' UTR in DNA? 5' UTR in mRNA? Shine-Dalgarno in DNA? Shine-Dalgarno in polypeptide? Promoter in RNA? Promoter in polypeptide sequence? Stop codon in mRNA? Stop codon in the polypeptide sequence? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] > <