A clinical test sometimes used to diagnose folate deficiency or B12 deficiency is a histidine tolerance test, where one injects a large dose of histidine into the bloodstream and then carries out a series of biochemical determinations. What histidine metabolite would you expect to accumulate in a folate- or B12-deficient patient, and why?

Q: A teenager was admitted to the hospital with extreme muscle pain, lightheadedness and fatigue after…

A: Cellular respiration is a metabolic process that is made up of three metabolic pathways: glycolysis,…

Q: Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If…

A: Dyslipoproteinemia is also known as dyslipidemia, which means the abnormal concentration of…

Q: Bacterial species that are capable of synthesising the amino acid histidine do not need it in their…

A: Introduction Histidine is synthesized from phosphoribosyl pyrophosphate (PRPP), which is made from…

Q: A species of bacteria can synthesize the amino acid histidine, so they do not require histidine in…

A: Feedback inhibition is a mechanism to control cellular activity. The activity of the enzyme is…

Q: Proteinase K should be used as the first step of DNA purification (before applying phenol CIA…

A: Proteins are large, complex molecules made up of chains of amino acids that perform various…

Q: How is the production of particular proteins limited to certain cell types at certain times,…

A: Fertilization of an egg cell by a sperm results in the formation of a zygote. The zygote undergoes…

Q: The mouse facility sends you the wrong knockout mice. For fun, you check levels of cholesterol and…

A: Knockout mice are created in the laboratory in which one or more genes have been deleted. Such mice…

Q: n the following scenarios, glucose is absent.  Judging these systems as a whole, decide if…

A: E. coli has an operon that codes for the breakdown of lactose. Within this lactose (Lac) operon,…

Q: Glucose 6-Pase is an enzyme present in liver cells but not in musc cells. The enzyme is bound to the…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: Polyphosphate (poly-P) Exopolysaccharides (EPS) • Autoinducers (AI) • Guanidine tetraphosphate…

A: Answers Rnase is an enzyme that is used to degrade the RNA molecules. While HipAB is a…

Q: The substrate which was used to investigate the enzymatic activity of wheat bran phosphatase was…

A: Base was added so that pH will become high as it can show the pink color. If pH will not be high…

Q: Failure of anti-oxidant function results in the hydroxylation of an aromatic acid of Enzyme Z and…

A: Sanger DNA sequencing is carried out using ddCCTP for the chain termination. The given peptide is…

Q: Many proteins are secreted in their inactive form. This is also true of many toxic proteins produced…

A: A precursor of a protein, also known as a pro-peptide or pro-protein, is an inactive form of a…

Q: Congenital sucrase-isomaltase deficiency (CSID) is a genetic condition that prevents the metabolism…

A:

Q: The Warburg effect is where tumor cells preferentially catabolize glucose to lactate even in the…

A: The Warburg Effect is known as an increase in the rate of glucose uptake and production of lactate,…

Q: HbA1c is used to monitor blood glucose levels because hemoglobin is the only protein in blood that…

A: “Since you have asked multiple question, we will solve the first question for you. If youwant any…

Q: Orotic aciduria results when one of the enzyme activities of UMP synthetase is absent. This syndrome…

A: Orotic aciduria is a hereditary disorder related to metabolism. Orotic aciduria is one of the…

Q: what are the molecular way to diagnose Lactose intolerance disorder ? describe in detail about…

A: Lactose is the main sugar in milk, which is a disaccharide. It is hydrolyzed by intestinal lactase…

Q: Is the following true or false? Biocatalysis is limited to the use of enzymes found naturally in…

A: Biocatalysts are substances used in biochemical reactions. The biocatalysts are used to increase the…

Q: Which of the following amino acid residues would not participate in general acid-base catalysis? A.…

A: Acid-base catalysis is the reaction mechanism that deals with the transfer of a proton from one…

Q: An individual with an argininosuccinase deficiency is administered benzoate and arginine. This…

A: Argininosuccinase deficiency is a condition where the cells are devoid of an important enzyme i.e.…

Q: What kinds of changes take place in a protein when it is denatured?

A: Denaturation is a process in which a protein loose the quaternary structure, tertiary structure, and…

Q: For each of the following genotypes, explain how mutation (identified by a (-) will affect the…

A:

Q: Why is it advantageous for two control mechanisms —allosteric control and covalent modification— to…

A: Enzymes are the proteins which act as a catalyst in chemical reactions. Enzymes never take part in…

Q: Define the following terms: a. enterocyte b. N-glycan c. O-glycan d. glycogen e. glycosaminoglycan

A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: imagine that you are running a sample on a TLC plate that is comprised of aspartate and isoleucine.…

A: The working principle of thin-layer chromatography is the same as column chromatography.A small…

Q: Idris has successfully extracted enzymatic proteins from the fish viscera (intestines and stomach).…

A: The first step after crude protein isolation from any sample is the quantification of the total…

Q: Protein kinases (PTKS) are enzymes that regulate the biological activity of proteins by…

A: CELL CYCLE ❅ The process by which a parent cell divides into two daughter cells. ❅ The cell cycle…

Q: Consider, for example, that a particular serine residue is phosphorylated to activate the protein.…

A: Phosphorylation can either activate or deactivate a protein. Phosphomimetics replaces amino acids in…

Q: between gene and protein was first articulated by Beadle & Tatum, who proposed the one-gene,…

A: The one gene–one enzyme theory proposes that genes work by making enzymes, with each gene producing…

Q: An extract of rat liver was prepared by homogenizing 65 g of tissue in 55 mL of buffer (Fraction 1).…

A: Given details in the problem: Fraction 1 is the initial fraction. Initial specific activity = 11.5…

Q: 1) Draw a chromatogram depicting separation of proteins 1, 2, and 3 (protein #1, mw 30,000, protein…

A: The diagram of chromatograph  is as follows-

Q: Sydney Brennen isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of…

A: By the use of minimal and deficit medium scientist can characterize different classes of mutants and…

Q: By definition, essential amino acids are not synthesized by an organism. Arginine is classified as…

A: Amino acids are the building blocks of amino acids which are broadly classified into two types based…

Q: A healthy immune system can raise antibodies that recognize and bind with high affinity to almost…

A: Catalytic antibodies are the one that have the potential to increase the rate of chemical reaction…

Q: The authors in the abstract given above describe the mechanism for the activation of metallothionein…

A: Protein Phosphatase 2A (PP2A) Binding: Complexes of PP2A PR110 bind to MTF-1, the metal regulating…

Q: Describe type I, II, and III L-asparaginases. Mention characteristics and differences between them…

A: L-Asparaginase Enzymes having antitumor activities. Degrade asparagine to aspartate with the…

Q: Suppose a new patient, patient HLU, presents with fatigue and failure to thrive within a few days.…

A: Hypoglycemia is a condition where the blood sugar level remains below normal. The normal blood…

Q: A dinical test sometimes used to diagnose folate deficiency or B,2 deficiency is a histidine…

A: Folic acid, vitamin B12, and vitamin C are responsible for the metabolism of proteins. Folate is…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.