Related questions
Question
Transcribed Image Text:Question 5 Electromagnetic phenomena and spectroscopy.
(a) A medical researcher is using the uniform electric field (E = 2400 NC-¹) generated
by an electrophoresis setup to isolate a charged protein of interest (q = +160e) from an
uncharged patient sample. As shown in panel (A), when the patient sample is loaded
between the positive and negative terminal, the charged protein of interest migrates 12
cm toward the negative terminal.
(A)
(B)
€€€
(ii)
(iii)
(iv)
(c)
+160e
-0
(1)
(ii)
(iii)
12 cm
= protein of interest
= patient sample
Intensity (a.u.)
100
80
60
40
20
ol
450
Excitation
spectrum
(b) Migration of the charged protein of interest was monitored by staining the patient
sample with a fluorescent dye that was specific for this biological molecule. The excitation
and emission spectrum of this fluorescent dye are presented in panel (B).
(1)
Emission
spectrum
500 550 600
Wavelength (nm)
How much work has the electric field done on the protein of interest?
What is the protein of interest's change in electric potential energy?
If the medical researcher wanted the charged protein of interest to migrate further
away from the uncharged patient sample, how could they achieve this?
What is the strength of the mass
spectrometer's magnetic field?
Is the magnetic field vector pointing into the
page or out of the page?
What is the mass of component A?
650
What wavelength of light (A) could be used to excite the fluorescent dye highlighting
the protein of interest within the electrophoresis gel?
What wavelength of light (A) could be used to detect the resulting fluorescence?
Calculate the photon energy associated with the wavelength of light (A) that you
suggest could excite the fluorescent dye.
Briefly describe why the emission spectrum of the fluorescent dye in panel (B)
occurs at longer wavelengths of light (A) compared to its excitation spectrum.
The extracted protein of interest is then subjected to mass spectrometry for
characterisation. As depicted in panel (C), when the protein enters the magnetic field of
the mass spectrometer with a velocity of 9 x 104 ms-1, two singly ionised components (A
and B) are detected 8 cm and 10 cm to the left of the point of entry. The mass of
component B is reported to be 7 x 10-23 kg.
(C)
2 cm
A B
0024
8 cm Protein of
interest
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by stepSolved in 3 steps with 7 images
Knowledge Booster
Similar questions
- Identical 42 μC charges are fixed on an x axis at x = ±3.4 m. A particle of charge q = –20 μC is then released from rest at a point on the positive part of the y axis. Due to the symmetry of the situation, the particle moves along the y axis and has kinetic energy 1.2 J as it passes through the point x = 0, y = 4.5 m. (a) What is the kinetic energy of the particle as it passes through the origin? (b) At what negative value of y will the particle momentarily stop?arrow_forward(a) Calculate the number of electrons in a small, electrically neutral silver pin that has a mass of 8.0 g. Silver has 47 electrons per atom, and its molar mass is 107.87 g/mol. (b) Imagine adding electrons to the pin until the negative charge has the very large value 3.00 mC. How many electrons are added for every 109 electrons already present?arrow_forward(a) Calculate the number of electrons in a small, electrically neutral silver pin that has a mass of 13.0 g. Silver has 47 electrons per atom, and its molar mass is 107.87 g/mol. (b) Imagine adding electrons to the pin until the negative charge has the very large value 1.00 mC. How many electrons are added for every 109 electrons already present?arrow_forward
- Two large, nonconducting plates are suspended 2.83 cm apart. Plate 1 has an area charge density of +92.3 µC/m², and plate 2 has an area charge density of +10.1 µC/m². Treat each plate as an infinite sheet. How much electrostatic energy UE is stored in 2.29 cm³ of the space in region A? UE = What volume V of the space in region B stores an equal amount of energy? V = J 3 cm³ Plate 1 Region A Region B Plate 2 Region Carrow_forwardA factory is spray-painting large sheets of metal electrostatically. The sheets have dimensions of 2.4 m by 2.4 m and are charged with 0.82 mC. A 10 kg drum of paint is charged with -89 μCμC, and the spray nozzle produces uniform droplets of 2.4 g. Assume that all charge is distributed uniformly on the sheet and in the paint and no charge is lost during the spraying process. Determine the magnitude of acceleration the paint droplets feel due to the metal sheet. Give your answer in units of ms2ms2.arrow_forwardGiven the magnitude of an electric field directed downward equals 100 N/C. If two point charges (qA and qB), qA=2000 V and qB= 1000V. The charges are located 10 cm apart. What is the field strength at qA?arrow_forward
- Ex:Region y 0 is a dielectric medium (€,r1 = 2). If there is a surface charge of p, = 2 n (а) А (3,-2,2) (b) В (-4,1,5) on the conductor, determine E and D at т2arrow_forwarda red blood cell contains 5.2x10^7 free electrons what is the total charge of these electrons in the red blood cell ?arrow_forwardTwo chloride ions and two sodium ions are in water, the “effective charge” on the chloride ions (Cl−) is −2.00 × 10−21 C and that of the sodium ions (Na+) is +2.00 × 10−21 C. (The effective charge is a way to account for the partial shielding due to nearby water molecules.) Assume that all four ions are coplanar. where a = 0.280 nm, b = 0.760 nm, and c = 0.460 nm. What is the direction of electric force on the chloride ion in the lower right-hand corner in the diagram? Enter the angle in degrees where positive indicates above the negative x-axis and negative indicates below the positive x-axis. ° Prevarrow_forward
arrow_back_ios
arrow_forward_ios