9. Complete the table by transcribing the following culture media, classify as to indication/function and consistency. Write letter for columns 3 and 4. 1 POINT PER SET OF CORRECT ANSWERS. Classification as to function: 1. ANA 2. APW 3. BAP 4. BCYE A. General isolation medium B. Enrichment medium C. Selective medium D. Selective differential medium E. Test medium CULTURE MEDIUM Anaerobic blood agar Classification as to consistency: A. Agar plate B. Agar deep C. Agar slant D. Broth CLASSIFICATION AS TO FUNCTION C CLASSIFICATION AS TO CONSISTENCY A
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- A researcher wants to compare the pathogenicity of a mutant pathogen relative to wild type in an animal model. The mutant is marked with the constitutive expression of a foc gene that turns colonies blue on X-gal agar. The input ratio of the experiment Dilue/white colonies) was 10:1. The output ratio of the infection experiment tant to d type was 1-100 a. What is the CI? Show your work. 1. Define the Cr and describe what it measures in your answer Which of these genotypes does better during infection, the mutant or the wild-type?The main reason/reasons that motivated the geneticengineers to use E. coli in the production of monoclonal antibodies compared to the usual techniques is/are: O a. The quality of monoclonal antibody produced by the usual method is poor. O b. The establishment of hybridomas is not easy. O. Theusual technique produces only polyclonal antibodies. O d. The isolation of lymphocytes is difficult. O e. The isolation of spleen cells is difficult.3 2. The table below was obtained by P1 phage transduction followed by media selection of an E. coli strain lacking the three genes. Determine the map order of the genes given the information provided. Demonstrate CLEARLY which are closer or farther from each other (no numbers needed). 1 2 MAP: Experiment Selected marker Ala+ Gly+ Ser+ Unselected markers (select- ed for by subsequent plating) 27% Ser+, 2% Gly+ 3% Ser+, 1% Ala+ 35% Ala+, 4% Gly+
- You perform a Kirby-Bauer assay with two antibiotics. Antibiotic X has a zone of inhibition of 9 mm. Antibiotic Y has a zone of inhibition of 11 mm. Which antibiotic is better at killing this particular microorganism? Group of answer choices 1Antibiotic Y 2Antibiotic X and Y, which have identical antimicrobial activities 3Antibiotic X4 4It is impossible to tell from the information givenThe following table includes information from a procedure where mice were injected with different concentration amounts of a certain microbe (Mircobe A) under these distinct circumstances: no other treatment added (NT), after being exposed to anti-microbe A vaccine (vacA), and during treatment with a broad-spectrum antibiotic (x). CFU/ml Injected % Infected - NT % Infected - vacA % Infected - x 1 2 0 1 10 20 0 22 100 52 3 44 1000 100 14 97 1. What is the ID50 value of Microbe A (in CFU/ml)? 2. Is Microbe A antibiotic-sensitive? 3. Is the vacA vaccine effective to stop the infection?The plaque assay plate below was made from a dilution of 10-7 and 0.1 ml of the dilution was plated on the cell monolayer. What is the virus titer in PFU/ml? Note: 10e1 means 10 to the first power, etc. Select all answers that apply.
- B ut of 1 e flag us page In Canada, facilities that participate in sterile compounding follow guidelines provided by USP Chapter , NAPRA and this professional association: Select one: O a. CAPT Q b. CPTEA O C. FDA O d. CSHP FS Q Search F6 - F7 F8 L F9 m |--- ■■■ 8 + Time left 21:19: F10 Next pageKatelyn had been working for Dr. Johnson for a month, and while she had become quite good at measuring inhibitionzones, she didn’t know why she was doing all this work. She had gotten very curious after she began doing all themeasurements on a new set of antibiotics. # is experiment involved infecting mice with MRSA and tracking how theMRSA grew over time.Data were collected by counting the cells of MRSA taken from $ uid samples from the mice. # e cells were measuredby taking one gram of the $ uid and spreading it over plates, but now Katelyn counted the colonies that grew on theplate after 24 hours. Because there were so many, she actually measured the colonies as “log CFU/g.” A CFU is acolony forming unit, or essentially a cell that will divide into a colony that can be seen. Because there can be so many,Katelyn measured them on a logarithmic (log) scale. # e raw data in her lab notebook looked like the following:Table 1. E% ect of treatment on MRSA in mice after 24 hours of drug…You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate? A. ~1.1 *10^6 PFU/mL B. ~1 *10^7 PFU/mL C. ~3 * 10^7 PFU/mL D. ~2 * 10^6 PFU/mL E. ~3.5 *10^6 PFU/mL F. ~1.1 * 10^5 PFU/mL G. ~3.5 *10^5 PFU/mL
- 5 strains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes, and only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media and colonies are observed the next day. Rich Media (Lb) E5 E4 E1 E2Ⓡ E3 MM + Mal+Leu E5 E4 X ● E1 E5 X MM + Mal E4 X X E1 E3 E2 E5 ✪✪ E2 E4 ● E1 E5 MM + Mal+Leu +Pro E2 E3 MM + Ara E4 X X E1 E3 E2 X X Colonies are indicated by a black dot, absence of a colony is indicated by a cross. 1) Determine the genotypes of the 5 bacterial strains (E1 - E5)Outline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.Describe and explain the difference in results if the serial dilution step of the T4 bacteriophage suspension inour experiment was skipped (i.e., all of Step #3 of the procedures was skipped)? _________________________Current EventsThe Johnson and Johnson COVID-19 vaccine consists of inactivated adenovirus that has been altered to containgenetic information encoding SARS-CoV-2 surface spike proteins. The virus targets and delivers this geneticinformation to the cells of our respiratory tract, which produce benign SARS-CoV-2 surface spike proteins butare effective for training our immune system to defend us against SARS-CoV-2.Based on this information and your knowledge of viruses in general, could T4 bacteriophage be used in place of