7. When doing Tissue Culture, it is essential that you understand how cells communicate with each other and how the environment communicates with the cells. Using a figure in your notes, describe how cells interact with their environment and other cells. What are we using in our media
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- Recall from the video the steps of how to make a wet mount of cheek epithelial cells. Place the steps of how to prepare a wet mount in the correct order from left • View Available Hint(s) Reset Help Add a drop of physiological saline to the center of the slide. Scrape the inside of the cheek with a toothpick. Stir the end of the toothpick in the physiological saline. Mix a drop of stain into the specimen preparation with a toothpick. Place a coverslip on top of the specimen.1. What substance is often applied on a slide specimen of cells or tissues that help enhance the appearance of cell parts under the microscope? 2. Name a very common, easily available, solution that is applied on onion cells or tissue to enhance their microscopic appearances?(The main benefit of a three-dimensional culture system is that: O the morphology and behavior of cells are closer to what they would be within the body. O it is easier to change the media in these systems. O more cells can be grown in three dimensions. O it does not have to be sterile. O they easily give rise to specific cell lines.
- When observing relatively thicker slices of tissue or specimens,.A. you may need to refocus slightly to see cells or structures that may be deeper ormore superficialB. you should focus on the most superficial layers of cellsC. you should focus on the deepest layers of cellsD. you should focus as closely to the middle of the specimen as possibleGrowing cells outside the body needs laboratory space, aseptic techniques and special equipments such as incubator. The terminology use to say growing cells in lab is calledWhat is the biological benefit(s) to the organism to have detachment induced cell death? Please explain as much as possible (at least 5 sentences)
- Staining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?Which of the following is an advantage of confocal microscopy over conventional fluorescence microscopy? The interaction of the laser beam with the cell surface allows the imaging of individual macromolecules O Only out-of-focus light enters the microscope lens, making the object appear illuminated against a dark background The use of electrons instead of light to image the specimen results in greatly increased resolving power O Optical sections in a single plane of focus can be visualized at different depths in the specimen The natural contrast of an unstained specimen is enhanced1) What is embedding? 2) What is the most common embedding medium for formalin-fixed tissues? 3) What is H& E staining and what does it stain?
- Stains are used because O they bind evenly to all str they reduce contrast and make it easier to see cells on a bright background they increase contrast and can be used to distinguish between types of structures or cells they increase resolution13. Compare the cells in the two photomicrographs below in terms of their shape and structure(s). А BHow do tissue cells look in comparassion to cheek cells using H&E staining?,.