5g. A mutation has occurred rendering the regulatory protein unable to recognize the operator sequence. What effect would this have on the expression of this operon? How would this impact the energetic demands on the cell? Justify your answer.

Kk.306.

ate) DEFINE what is an Operon, and compare and contrast regulation of the LAC Operon and the TRP Operon. When is each turned ON or OFF? A. DRAW each operon in the PRESENCE of its own ligands (signal molecules). B. What controls the activity of the regulatory proteins involved? · Be sure to describe and show both POSITIVE and NEGATIVE regulation where appropriate. C. Explain HOW each type of control system is appropriate for an operon encoding either catabolic or anabolic enzymes.

1. The table below shows partial diploid E. coli with lac operon mutations For each mutant, indicate whether expression of permease is:• None (absent)• Basal (on but low expression)• High (on and activated expression)when grown in either lactose only or glucose only media. Also indicate the cell’s overall growth phenotype (lac+ or lac-) when grown in lactose only and why you expect that phenotype (provide “why” for ONLY THREE of the mutants). ***Recall that a bacterial cell needs at least one functional copy of lacZ AND lacY expressed at high levels to producean overall growth phenotype of lac+ when grown in lactose only media. Lac Mutant CAP bindingsite Expression inlactose only Expression inglucose only Phenotype in lactose only I+ P+ O+ Z- Y+ / I- P+ O+ Z+ Y- WT       Why?: Why?: Why?: Why?: Why?: I+ P+ O+ Z- Y- / I+ P- O+ Z+ Y+ WT       Why?: Why?: Why?: Why?: Why?: I+ P+ O+ Z- Y+ / I+ P+ OC Z- Y+ WT       Why?: Why?: Why?: Why?: Why?: IS P+ O+ Z+ Y- / I- P+ O+ Z+…