4. Why is it important to keep the temperature below 10C in the synthesis of methyl orange experiment.

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For the reaction of nitrogen and oxygen to form nitrogen dioxide,                                              N2(g) + 2O2(g) ⟷⟷    2NO2(g)              Kc = 1.5×103 If the reaction mixture is found to contain 2.0 M NO2 and 0.10 M N2 and 0.10 M O2, what is the value of Qc and what does this mean? A)Qc = 4×103  B)Rxn will shift to the products C)Qc = 2×102 D)Rxn will shift to the reactants

2. As you are probably aware, some proteins "glow," most famously Green Fluorescent Protein (2008 Nobel Prize in Chemistry). The color of these proteins can be shifted by an elimination reaction as described in the article "The E1 Mechanism in Photo-Induced B-Elimination Reactions for Green-to-Red Conversion of Fluorescent Proteins." NRVF. NRVF. His-Nu H N-ATTL. KİKGRX ATTL. HO NH HO His-Ce hv His-CB a) Propose a simple mechanism for the reaction using arrow pushing. b) How might His-Na (which sits in the surrounding protein) accelerate the reaction? To initiate the reaction, should this histidine be in its acid or conjugate base form? Draw this form of histidine side chain. c) How about His-CB? d) Provide a brief explanation of why the reaction yields a shift from green to red color.