4. What is the effect of heat on an enzyme? 5. What do you predict would happen in the reaction in cold temperatures? 6. What is the effect of pH on an enzyme? What is the best pH for an enzyme?

22.  What is a major benefit of calculating catalytic efficiencies? Group of answer choices It determines whether a catalytic reaction is cooperative It allows you to compare different substrate preferences for an enzyme It tells you the rate of an enzyme reaction It allows you to determine the efficiency of the enzyme being studied

2. Draw a graph showing a downhill (spontaneous) reaction, that requires some activation energy to get it going. Then draw and clearly label on the graph b. The activation energy for the reaction (use a bracket to indicate the upper and lower limits) c. The free energy change for the reactions (use a bracket to indicate the upper and lower limits) d. How an enzyme will change the energetics of the reaction. I suggest you use a dotted line for the enzyme.

8 eBook Select all of the following that could apply to the brown curve. Enzyme concentration is higher. Temperature for an enzyme is too high. Temperature for an enzyme is optimal. Activation energy is lower. Enzyme concentration is lower. Activation energy is higher. Reactants Progress of reaction, Products

45.  Which of the following is/are true? (MARK ALL THAT APPLY) Group of answer choices Enzymes lower the activation energy of the reaction Enzymes bind more strongly to the substrates than the transition state of the reaction Enzymes alter the standard free energy of the reaction. Enzymes force reactions to proceed in only one direction. Enzymes alter the equilibrium of the reaction.      PreviousNext

3. At what substrate concentration would an enzyme with a keat of 25.0 s¹ and a KM of 3.5 mM operate at 25% of its maximal rate? How many reactions would the enzyme catalyze in 45 seconds when it is fully saturated with substate, assuming the enzyme has one active site?

14) If you performed a similar experiment to test the effects of pH on the activity of the enzyme pepsin, what would you predict to be optimal pH of pepsin? Why?

20) Normally an enzyme has maximal enzyme activity at 37°C, but once it has been heated to 100°C and then cooled back down to 37°C the enzyme is no longer active. Why?

1. Based on the graph, which of the following could be used to increase the reaction rate beyond point C? a. increase the amount of substrate b. decrease enzyme concentration C. add more water d. increase the temp 2. Which explanation best fits the graph for pH's effect on an enzyme? Substrate concentration a.As the pH gets closer to neutral more H-Bonds are broken b.Larger numbers of H+ & OH- ions distort the active site c. There are more collisions between substrates and active sites near the optimal pH d.Low pHs cause more H-Bonds to happen near the active site Optim pll

19. What is the optimal pH for catalase? Why do you think it becomes less effective at high or low pH? 20. Can you think of anywhere in the human body where enzymes would work BEST at a high pH? Please give an example and draw what that graph might look like. 21. What is the optimal temperature for catalase? Why do you think it becomes less effective at high or low temperatures? 22. Can you think of any organisms that would have enzymes specifically adapted to extreme low or high temperatures and would therefore function best at a different part of the graph? Please give an example and draw what that graph might look like.