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- 1. How many TSS sites were identified using this technique? 2. Look at the labels next to each of the annotated TSSs. What are the labels for the TSS sites? 3. What is the coordinate for TSS_tra_16584216?Outline the 7 steps of 1H NMR.A. Basic mechanism of 2d gel electrophoresis techniqes? B. How do we predict the protein structure from DNA sequence?
- 1. Why were nuclei being unstained when you used eosin? 2. What is the relationship between heterochromatin and the synthetic activity of DNA? 3. What is the significance of using different histological stains? 4. Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and nucleic acid? What are the differences? 5. How essential is Periodic Acid- Schiff Reagent? please answer with 3-5 sentencesWhy are appropriate transducer orientation and image standardization important in vascular sonography?5. Show the separation pattern of the following DNA molecules on an agarose gel electrophoresis. 5 kbp 5 kbp 5 kb 5 kbp ----
- How does magnetic bead DNA extraction work?What are dyes that stain DNA used for? Is background staining a problem when using such dyes?13 Match each of the QPCR samples (i-v) with the correct amplification plot (A-E) and determine the Ct value for each of the amplification curves. Number of template copies in the QPCR reaction: Amplification plot (A-E): Ct Value 1200 1000 i. 1X 106 ii. 1X 105 iii. 1X 108 iv. 1X 107 V. NTC (No template control) 800 600 400 200 A C 0 5 10 15 20 25 30 Cycles vi. Create a standard curve graph of template copies vs Ct with the data in the table above. Log of Starting Quantity of DNA vrs C+ vii. 26 24 22221 20 18 16 14 12 כי 10 8 6 4 2 0 5 5.5 6 6.5 7 7.5 8 Log Starting Quantity of DNA (Template copies) If a patient sample has a Ct of 20, interpolate the number of template copies at the start of the PCR reaction using the standard curve you created above?
- With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the optical system used within the Nanodrop?Why do we need to amplify the DNA at all in order to visualize it in a gel?what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp DNA fragment and why?
