4. Researchers were investigating the effect of various factors on the rate of mitosis. MPF (Mitosis Promoting Factor) is thought to cause more cells to begin mitosis in human skin cells. To test this hypothesis, human skin cells were cultured with plain nutrient media and with nutrient media to which MPF was added. After 24 hours, cells were sampled, stained and observed under the microscope to see whether they were in mitosis or interphase. The data obtained is recorded below. Use the data to evaluate whether MPF does cause more cells to enter and thus be in mitosis. (Follow the steps as in problem #1 on a separate sheet of paper.) THINK: How will the data in the middle column be useful to you? The Effect of MPF on the Division of Human Skin Cells Cells Grown without MPF Skin Cells in Mitosis Skin Cells in Interphase 205 1795 Cells grown with MPF 493 2082

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