4) Apply Beer - Lambert law, to calculate the conc. For Fluorescein using the below data and the suitable (ɛ) value from your manual. For Fluorescein: A= 0.298 at A = 495 nm For Fluorescein: A= 0.276 at A= 500 nm 0.6- 0.5- 0.4- 0.3- 0.2- 0.1- 0.0- 400 500 600 Wavelength (nm) Abs 0 298 0275

4) Apply Beer - Lambert law, to calculate the conc. For Fluorescein using the below data and the suitable (ɛ) value from your manual. For Fluorescein: A= 0.298 at A = 495 nm For Fluorescein: A= 0.276 at A= 500 nm 0.6- 0.5- 0.4- 0.3- 0.2- 0.1- 0.0- 400 500 600 Wavelength (nm) Abs 0 298 0275

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

4) Apply Beer - Lambert law, to calculate the conc. For Fluorescein using the below data
and the suitable (ɛ) value from your manual.
For Fluorescein: A= 0.298 at A = 495 nm
For Fluorescein: A= 0.276 at A = 500 nm
%3D
0.6-
0.5
0.4
0.3
0.2-
0.1
0.0
400
500
600
Wavelength (nm)
Abs
0 298
0275

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