4) Apply Beer - Lambert law, to calculate the conc. For Fluorescein using the below data and the suitable (ɛ) value from your manual. For Fluorescein: A= 0.298 at A = 495 nm For Fluorescein: A= 0.276 at A= 500 nm 0.6- 0.5- 0.4- 0.3- 0.2- 0.1- 0.0- 400 500 600 Wavelength (nm) Abs 0 298 0275

4) Apply Beer - Lambert law, to calculate the conc. For Fluorescein using the below data and the suitable (ɛ) value from your manual. For Fluorescein: A= 0.298 at A = 495 nm For Fluorescein: A= 0.276 at A= 500 nm 0.6- 0.5- 0.4- 0.3- 0.2- 0.1- 0.0- 400 500 600 Wavelength (nm) Abs 0 298 0275

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: C3H7OH ), known as rubbing alcohol, is ________ amu (rounded to one decimal place). Group of answer…

A: Molar mass of the compound can be calculated by multiplying atomic mass of each atom in the molecule…

Q: 1E. C₂H12O₂

A: Given Molecular formula: C8H12O2 To Determine: 1) IHD 2)Label the significant peak 3) Determine the…

Q: concentration

A: According to the question, the concentration of ascorbic acid is ~0.001 M in lemon juice, We need to…

Q: An empty erlenmeyer flask weighs 241.3 g. when filled with water (density - 0.997 g/mol), the flash…

A: Given: Mass of empty Erlenmeyer flask = 241.3 g. Mass of Erlenmeyer flask with water = 489.1 g. And…

Q: Express your answers to at least 5 significant figures. The mass of one mole of boron is 10.811 g.…

A: Using mole concept easily determines number of atoms and molecules present in one mole of substance.

Q: Which of the following is the best reducing agent? O Ba2+ O Ca(s) O Mg2+ O Mg(s) O Ba(s)

A: We have to choose the best reducing agent among the following given options as follows in step 2:

Q: If the density of ethanol is 0.789 g/mL, how many cm3 can be produced by the fermentation of 45.4 kg…

A: Ethyl alcohol is formed by enzyme action on sugars and starches by fermentation. C6H12O6 → 2 CO2 + 2…

Q: If sucrose costs $1.20 per pound, and a bottle contains 2.00 kg, how much would you pay for a case…

A: we know that, kg and pound are related ro each other as;2.205 pounds=1 kilogram1 pound=12.205*1…

Q: 9.921*10^-20*9.714*10^-19]^2

A: Given: [9.921 X 10-20 X 9.714 X 10-19]2

Q: 4. How sizing down to Nano affect material properties?

A: Since substances at the nanoscale level have a greater surface-to-volume ratio, which causes them…

Q: If you have 10 pieces of bread, 20 slices of ham, and 4 slices of cheese, how many sandwiches can…

Q: We have 425 cm3 of a material. How many g is it? The material has density of 0.345 g/cm O 68.0 g O…

A: We have to find mass ,volume and density is given in the question Volume of the material = 425 cm3…

Q: One of the reactions that occurs in a blast furnace, in which iron ore is converted to cast iron, is…

A: Answer: In this question we need to convert mass of Fe obtained into mass of Fe2O3 by using…

Q: What is the volume, in cubic centimeters (cm^3) of 1.00 kilograms of gold?

Q: 2. The Anemovax COVID-19 vaccine uses 0.950% (w/v) NaCl (normal saline, preservative- free) as its…

Q: Deterioration of buildings, bridges, and other structures through the rusting of iron costs millions…

Q: One way the U.S. Environmental Protection Agency (EPA) tests for chloride contaminants in water is…

A: Hi, as you have posted 2 questions and have not mentioned which one you want us to solve for you.…

Q: (MC) A student who likes the taste of salt adds 1.50 moles of sodium chloride (table salt) to her…

A: Given : Moles of sodium chloride i.e NaCl = 1.50 mol.

Q: A chemist prepares a solution of potassium iodide (KI) by weighing out 0.5448 kg of potassium iodide…

Q: NaNO3(aq) + H20(1) → NO(g) + O2(g) + NAOH(aq)

A: Here the elctrosysis of NaNO3 is done by passing 90A current for 77.2 minutes. We have to determine…

Q: What minimum amount of KO2 is required for the apparatus to produce enough oxygen to allow the user…

Q: Question 11: You are a gemstone expert studying samples of various materials. Gemstone Hambergite…

A: From the given gemstones, the most massive gemstone is = Smoky Quartz (density = 2.65 g/mL) The…

Q: What is the relationship between calculating the amount of iron in a vitamin pill and physical…

A: The analysis of present iron in a supplement tablet can be done by a redox titration reaction. Iron…

Q: What would be the mass, in grams, of a sphere of titanium which is 24.68 mm in diameter? V =…

A: Given : Sphere of titanium with diameter = 24.68 mm = 2.468 cm…

Q: the percentage purity of the potassium cyanide.

Q: individual bikes to work one day a week 50/52 work weeks (5 days) a year. they save 12.5

Q: In preparing a zinc oxide ointment, 28.35 g of zinc oxide was used rather than the correct…

Q: a Assume that mitochondria are cylinders 8.00 um in length and 0.800 pm in diameter. What is the…

A: According to the bartleby guidelines for the multiple questions. I can solve only first question.

Q: Calculate the cost of one pound of nitrogen from a f

A: The objective of the question is to determine the cost of one pound of nitrogen from a fertilizer…

Q: 1. CH 3 μg Br 2. H+

A: The given reactant is an epoxide. When an epoxide reacts with a Grignard reagent, an alcohol would…

Q: Is this an Endothermic Sn1 diagram?

Q: Give an example of a mixture in which solute is solid and solvent is liquid.

A: NaCl (ionic solid) Water (liquid) Salt water

Q: Write the different methods of Equivalence Calculations?

A: There are various methods of finding equivalence point. Few of them are as follows: 1) Color change…

Q: Chemistry 1) CH3 CH3 FHO- Br 2) CH3

Q: A chardonnay wine is 13.5 percent by mass alcohol. If we consume 24 fluid oz of wine, and the…

A: Percent by mass alcohol = 13.5 % Volume of wine = 24 oz 1 oz = 29.57 mL 24 oz = 709.76 mL or 0.70976…

Q: To aid in the prevention of tooth decay, it is recommended that drinking water contain 0900 ppm…

A: Given 0.900 ppm = 0.900 g/106 cm3 water dimensions of cylindrical water reservoir diameter = 5.29 x…

Q: 1 Solutions of cyanide salts are still widely used in the extraction of gold metal from its ores. It…

A: Two numerical problems based on extraction of ore, which are to be accomplished.

Q: Mixtures are created by physical means (mixing a substance with another; no chemical bonding); if…

A: A mixture is a material containing two or more elements or compounds that are in close contact and…

Q: d. e. OCH3

A: Coupling reaction: Diazonium salt show coupling reaction with phenols aniline, ethers at ortho…

Q: 1. O3 2. Zn, H,0*

A: The reaction product and the reagents given are,

Q: Calculate the number of atoms in 264. ug of cadmium. Be sure your answer has the correct number of…

A: Given:Mass of cadmium (Cd) = 264 g = 264 x 10-6 g = 2.64 x 10-4 gWe have to determine the number of…

Question

4) Apply Beer - Lambert law, to calculate the conc. For Fluorescein using the below data
and the suitable (ɛ) value from your manual.
For Fluorescein: A= 0.298 at A = 495 nm
For Fluorescein: A= 0.276 at A = 500 nm
%3D
0.6-
0.5
0.4
0.3
0.2-
0.1
0.0
400
500
600
Wavelength (nm)
Abs
0 298
0275

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

What are advantages and limitations of different types of modelling? (modelling with experiments, computer simulations, modelling kits and quantitative modelling)
Q1. The net potential energy between two adjacent ions, EN, is represented by A В E N pn where EN is the net energy in Joule (J), A and B are constants with values of 1.436 J.nm and 7.32 × 106 J.nm, r is the interatomic distance between the two ions in nanometer (nm), and n is a positive number with a value of 8.
4- Give any branch of chemistry that is widely studied? Chemistry is the study of matter - anything that has mass and volume. This central science looks into the physical and chemical properties of matter and how these properties are connected to the structure of matter in an atomic or molecular level. The changes involved in matter are also looked at be it physical change or chemical change.
5-
Which piece of equipment would you use to measure 7.10 mL of CuSO4 (aq)?
3. O Be CH₂CH₂ KW дках 2) 1₂0 HO: CHI 14. M Soll Pyr 2
Complete the following nuclear equations. Write the mass number, atomic number, and symbol for the remaining nuclide. (Enter your answer in the form X. Enter He for an alpha particle, 18 for a beta particle, y for a gamma particle, 8 for a positron, n for a neutron, and p for a proton.)
Scientists can learn a great deal about a community's drug use (both legal and illegal) by analyzing the contaminants in wastewater. In a recent study in New York State, chemists measured the concentration of cotinine, a derivative of nicotine that i produced in the liver and excreted in the urine of tobacco users. The chemists found the average cotinine concentrations in the wastewater stream was 1.43 µg/L. a Ι Ι H- b HH H H-C C-N: C=0: TH HH Jonutis/Shutterstock Revell, Introductory Chemistry, 2e, © 2021 W. H. Freeman and Company Cotinine is slightly soluble in water. Using the structure of cotinine, identify any polar covalent bonds that are present in this substance. C-O C-C C-H C-N
ча OH A OH D B с CHO
The world population is estimated to be 7.4 x 109. Nauru is the smallest island nation and comprises 1.5 x 10-4% of the world population. If the percentage of left-handed people is approximately 12%, estimate the number of left-handers on the island of Nauru.
P1