3. What is the molecular formula of sucrose? (in 1 to 3 sentences, explain whythis is your answer).a. C12H22011b. C,,H20010c. C,H,,06d. C1„H2,0114. The glycosidic linkage between glucose molecule in maltose is? (in 1 to 3sentences, explain why this is your answer).а. В 1 -4b. a 1 - 2с. В 1 -4d. a 1 - 2

Sucrose: It is a disaccharide sugar composed of glucose and fructose monomers. C1 of glucose monomer…

Answered: 3. What is the molecular formula of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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10. Chymotrypsin is a serine protease enzyme. The Km for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8*102, and the Km for the reaction of chymotrypsin with N- acetyltyrosine ethyl ester is 6.6*10“ M. catalytic triad Ser 195 His 57 Gly 193 N-H OH R-N- Ca N-H O-C- Asp 102 N-acetyl valine N-acetyl tyrosine Chymotrypsin Active Site a. What is the nucleophile here and how is it activated? b. Which substrate has an apparent higher affinity for the enzyme. c. Propose a reason for the difference in affinity based on the shape of each of the substrates (see active site figure, cleaves on the C-side of aromatic residues).
2B. S. aureus hemolysin B attacks the RBC cell membrane by hydrolyzing the sphingomyelin headgroup: ОН HN .R hemolysin B cuts this bond i) Draw a plausible mechanism of hydrolysis for this lipid headgroup. Let B- and BH be general base and general acid. 00-P-O LOR2 OR, ii) Why is this damaging to the overall membrane architecture of the RBC?
4. The glycosidic linkage between two glucose molecules in isomaltose is? Explain your answer in 1-3 sentences. a. a 1 - 4 b. В 1 4 С. а 1 d. В 1 -6 5. Which of the following is an alpha lactone? Explain your answer in 1-3 sentences. a. Vit C b. Vit D c. Vit A d. Vit K
3. Solve the sequence of an oligopeptide 7 residues long which gave: Asp Leu Lys Met Phe Tyr The following facts were observed: a. Trypsin treatment had no apparent effect b. The PTH derivative from Edman degradation was c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met. d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys.
Match the following nucleobases (designated AD) with their names and answer questions about them. NH, NH, C D NH2 Note that some of the items from the answer list should NOT be used. Guanine 1. Aand B - Cytosine 2 Cand D • Adenine 3. Not shuwn v Thymine 4. D v Uracil 5. B a Aand C v Which of tho shown bases are pyrimidines? 7. A and O v Which of the shown bases form canonical A-T Watson-Crick base pair? S. A v Which of the shown bases form canonical G-C Watson.Crick base-pair? 9. Band C 10. C 11. Band D
3. Solve the sequence of an oligopeptide 7 residues long which gave: Asp Leu Lys Met Phe Tyr The following facts were observed: a. Trypsin treatment had no apparent effect b. The PTH derivative from Edman degradation was c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met. d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys. Instructions Make use of the table below to determine the sequence of the mystery protein.
1. At pH 7, the net charge of lysine is A. -2 В. -1 С. О D. +1 Е. +2 2. All 20 amino acids have A. one chiral carbon В. one a-carbon C. one carboxyl group D. ionizable R group E. neutral charge at physiological pH 3. The a helix structure of a polypeptide is maintained by A. peptide bonds B. van der Waal's force C. hydrogen bonds D. ionic bonds E. disulphide bonds 4. Which side chain of the following amino acid is deprotonated at pH 9? A. Methionine B. Alanine C. Isoleucine D. Histidine Е. Тyгosine 5. How many chiral carbons are in the following sugar? А. 1 В. 2 С. 3 D. 4 Е. 5
9. If 32p-labeled inorganic phosphate were introduced to erythrocytes undergoing glycolysis which of the following molecules would you NOT expect to detect 32p in? ** Multiple answers: Multiple answers are accepted for this question a. Fructose 1-6 bisphosphate b. 3-Phosphoglyceraldehyde c. Dihydroxyacetone phosphate d. 2-Phosphoglycerate e. 1-3 bisphosphoglycerate f. Phosphoenolpyruvate g. 3-Phosphoglycerate h. Pyruvate i. Glucose 6-Phosphate
8. The brick red color formed in a positive result of Benedict's test comes from A. the oxidation of copper ions B. the reduction of copper ion C. the reduction of carbonyl group D. the oxidation of carboxyl group E. the breakdown of glycosidic linkage 9. The following molecule is a но H Н-С—N — С H H H H-N-C -C-0-P-0-C -H H H H H A. triacylglycerol B. steroid hormone C. phospholipid D. o-6 fatty acid E. cholesterol 10. Which of the following statements about nucleic acid is INCORRECT? A. Coenzyme A is a protein, not nucleic acid B. NAD is an example of nucleic acid C. Adenine base is the same in ribonucleotide and deoxyribonucleotide D. Helix is an example of the secondary structure of nucleic acid E. Hydrogen bond can be found in DNA 11. The nucleoside consists of a D-deoxyribose and a cytosine base is called A. cytidine B. deoxycytidine C. deoxycytidylate D. deoxyribosylcytosine E. deoxyribosylcytidine
8) Consider the tetrasaccharide stachyose drawn below. Stachyose is found in white jasmine, soybeans, and lentils. Because humans cannot digest it, its consumption causes flatulence. HO How ОН OH stachyose OH HO HO OH HO OH OH OH OH «ОН a. Label all glycoside bonds. b. Classify each glycosidic linkage as a or ẞ and use numbers to designate its location between two rings (e.g., 1-4-3). c. What products are formed when stachyose is hydrolyzed with H,O"? d. Is stachyose a reducing sugar? e. What product is formed when stachyose is treated with excess CHI, Ag₂O? f. What products are formed when the product in (e) is treated with H₂O"?
9. A pentose sugar reported to be present in heart cells (in 1 to 3 sentences, explain why this is your answer). а. хуlose b. arabinose с. [ухose d. xylulose 10. Which of the following is an epimeric pair? (in 1 to 3 sentences, explain why this is your answer). a. D-glucose and D-mannose b. D-glucose and D-galactose c. D-glucose and L-glucose d. both A and B
a. Why is it important to eat food containing antioxidants? Write at leasttwo reactions to prove the answer. b. Write in four ways the following nucleotide sequence: ATGCA. c. Explain i. Hoogsteen pairing & ii. Hyperchromic effectiii. Epimers Iv. Mutarotaton v. Aldose vi. Anomers vii. Mutarotation

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